Anticancer potentiality of lignan rich fraction of six Flaxseed cultivars
Ezzat, Shahira M; Shouman S.A.; Elkhoely A.; Attia Y.M.; Elsesy M.S.; El Senousy A.S.; Choucry M.A.; El Gayed S.H.; El Sayed A.A.; Sattar E.A.; El Tanbouly N.
Date issued:
2018
Publisher:
Nature Publishing Group
Series Info:
Scientific Reports
8
Type:
Article
Keywords:
antineoplastic agent
,
lignan
,
plant extract
,
tumor marker
,
animal
,
apoptosis
,
chemistry
,
drug effect
,
experimental mammary neoplasm
,
female
,
flax
,
genetics
,
HCT 116 cell line
,
HeLa cell line
,
human
,
MCF-7 cell line
,
metabolism
,
mouse
,
Animals
,
Antineoplastic Agents
,
Apoptosis
,
Biomarkers, Tumor
,
Female
,
Flax
,
HCT116 Cells
,
HeLa Cells
,
Humans
,
Lignans
,
Mammary Neoplasms, Experimental
,
MCF-7 Cells
,
Mice
,
Plant Extracts
Abstract:
The objective of our study is to highlight the therapeutic effect and mechanism of action by which purified Flaxseed hydrolysate (PFH) which is a lignan rich fraction exerts its anticancer activity on a human breast cancer cell line (T47D) and in mice bearing tumor. HPLC analysis of PFH of six flaxseed cultivars had shown that PFH of the cultivar Giza 9 (PFH-G9) contains the highest concentration of SDG (81.64 mg/g). The in vitro cytotoxic potentiality of PFH's of six flaxseed cultivars was screened against a panel of human cancer cell lines. PFH -G9 showed the most significant cytotoxic activity against ER-receptor positive breast cell lines MCF7 and T47D with IC50 13.8 and 15.8 ?g/ml, respectively. Moreover, PFH-G9 reduced the expression of the metastasis marker, 1-?, metalloproteinases and vascular endothelial growth factor (VEGF), one of the most potent stimulators of angiogenesis, while it increased the caspase-3 dependent apoptosis. Our study also showed that dietary intake of 10% of Giza 9 Flaxseeds (FS), fixed oil (FSO) or Flax meal (FSM) twice daily for 3 weeks in mice-bearing solid Ehrlich ascites carcinoma (EAC) resulted in reducing the tumor volume, the expression of estrogen, insulin growth factor, progesterone, VEGF and MMP-2, but enhanced expression of caspase-3. � 2018 The Author(s).
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