dc.contributor.author |
Shahin, Hady |
|
dc.contributor.author |
Belcastro, Luigi |
|
dc.contributor.author |
Das, Jyotirmoy |
|
dc.contributor.author |
Grigoriadi, Marina Perdiki |
|
dc.contributor.author |
Saager, Rolf B |
|
dc.contributor.author |
Steinvall, Ingrid |
|
dc.contributor.author |
Sjöberg, Folke |
|
dc.contributor.author |
Olofsson, Pia |
|
dc.contributor.author |
Elmasry, Moustafa |
|
dc.contributor.author |
El-Serafi, Ahmed T |
|
dc.date.accessioned |
2024-04-06T15:27:51Z |
|
dc.date.available |
2024-04-06T15:27:51Z |
|
dc.date.issued |
2024-03 |
|
dc.identifier.other |
https://doi.org/10.3389/fbioe.2024.1328504 |
|
dc.identifier.uri |
http://repository.msa.edu.eg/xmlui/handle/123456789/5927 |
|
dc.description.abstract |
Introduction: The role of Adipose-derived mesenchymal stem cells (AD-MSCs) in skin wound healing remains to be fully characterized. This study aims to evaluate the regenerative potential of autologous AD-MSCs in a non-healing porcine wound model, in addition to elucidate key miRNA-mediated epigenetic regulations that underlie the regenerative potential of AD-MSCs in wounds. Methods: The regenerative potential of autologous AD-MSCs was evaluated in porcine model using histopathology and spatial frequency domain imaging. Then, the correlations between miRNAs and proteins of AD-MSCs were evaluated using an integration analysis in primary human AD-MSCs in comparison to primary human keratinocytes. Transfection study of AD-MSCs was conducted to validate the bioinformatics data. Results: Autologous porcine AD-MSCs improved wound epithelialization and skin properties in comparison to control wounds. We identified 26 proteins upregulated in human AD-MSCs, including growth and angiogenic factors, chemokines and inflammatory cytokines. Pathway enrichment analysis highlighted cell signalling-associated pathways and immunomodulatory pathways. miRNA-target modelling revealed regulations related to genes encoding for 16 upregulated proteins. miR-155-5p was predicted to regulate Fibroblast growth factor 2 and 7, C-C motif chemokine ligand 2 and Vascular cell adhesion molecule 1. Transfecting human AD-MSCs cell line with anti-miR-155 showed transient gene silencing of the four proteins at 24 h post-transfection. Discussion: This study proposes a positive miR-155-mediated gene regulation of key factors involved in wound healing. The study represents a promising approach for miRNA-based and cell-free regenerative treatment for difficult-to-heal wounds. The therapeutic potential of miR-155 and its identified targets should be further explored in-vivo. |
en_US |
dc.description.uri |
https://www.scimagojr.com/journalsearch.php?q=21100835954&tip=sid&clean=0 |
|
dc.language.iso |
en |
en_US |
dc.publisher |
Frontiers Media S.A. |
en_US |
dc.relation.ispartofseries |
Frontiers in Bioengineering and Biotechnology;Volume 122024 Article number 1328504 |
|
dc.subject |
adipose-derived mesenchymal stem cells; fibroblast growth factors; miR-155-5p; miRNA; porcine wound model; proteome; wound healing |
en_US |
dc.title |
MicroRNA-155 mediates multiple gene regulations pertinent to the role of human adipose-derived mesenchymal stem cells in skin regeneration |
en_US |
dc.type |
Article |
en_US |
dc.identifier.doi |
https://doi.org/10.3389/fbioe.2024.1328504 |
|
dc.Affiliation |
October University for modern sciences and Arts MSA |
|