Abstract:
Honey, rich with polyphenols, vitamins, proteins, organic acids and minerals, and is supersaturated solution of sugars.
Gamma irradiation could be a physical food preservation technique which protects against food related-insects and microbial
contamination and increases the shelf life of some products. The present research was investigated the efect of γ-irradiation
(0.0, 2.5, 5.0 and 10.0 kGy) on the physicochemical possessions of the substances of honey; phenolic and favonoids content
in addition to the antioxidant activity by DPPH radical. Diastase No. is also identifed with and without gamma irradiation.
After γ-irradiation a minor decrease in moisture content was observed while color intensity was signifcantly increased by
elevating irradiation dosage height mainly dose intensity 10.0 kGy which gave the premier color intensity compared with
control. But there was no signifcant diference in the pH values and total soluble solids (TSS%) as well as sugars% of
honey samples. Signifcant increases within the phenolic content were observed with the use of γ-rays particularly at dose
10.0 kGy which gave the main content relative to the control, while a trivial increase in the favonoid content was obtained
within the rise of the γ-irradiation dose. The fndings have also shown that free radicals can be scavenged by honey and
reveal high activity of antioxidants. Hydroxymethylfurfural is reduced by raising the irradiation dose, in converse diastase
No. is enlarged by amplifying the level of the γ-rays. Also, the results showed that the inhibition zones varied according to
the irradiation dose level, the dose level 10.0 kGy was more efective for inhibiting the growth of both Gram-positive and
Gram-negative bacteria as well as fungal development. The lung cancer cells (A549) showed a decrease in cell viability and
density, irradiated honey with dose level 10.0 kGy gave the lowest IC50 (6.08 mg/ml). Honey treated with a dose of 10.0 kg
of gamma rays did not afect the physical and biochemical properties of honey and was more efective as an antimicrobial
and anti-lung cancer, and the quality of honey remained unchanged upon radiation treatment.