Abstract:
The pollution of water became an international concern. Regardless which purpose water
is used, it should not be contaminated by pathogenic microorganisms or harmful heavy
metals. Chitosan nanoparticles (CNPs) have been considered potential antibacterial agents
effective against a large variety of microorganisms. In addition to graphene oxide which
plays a critical role in removal of heavy metals beside its antimicrobial activity. The
biocompatible polymer mixed with the GO NPs led to low cytotoxicity on human cells,
uniform size and excellent stability. This research mentions a critical estimation of the
contribution of the mixed GO\CS nanomaterials in water treatment and analyze the
physical, chemical and microbiological properties of surface water taken from several sites
in Egypt (temperature, pH, turbidity, ammonia, Phosphorous. aeruginosa, Vibrio, and
Flavobacterium) and thus improve these values using the nanocomposite. Water
examination were done by chemical and heavy metal examination by ICP-OES elemental
analysis technique and bacteriological examination of water and fish samples by a
molecular identification of isolated bacteria through PCR and sequencing. GO\CS
nanocomposite have been prepared by mixing the aqueous solution of chitosan and
graphene oxide in the present of diluted acetic acid and the characterization of nanomaterial
achieved by using TEM. The water examination was conducted and the result shows that
samples were contaminated with Cu, Cd, and Zn. Graphene oxide and Chitosan
nanocomposite was successfully prepared and added to the water sample which contains
Staph.spp., Pseudomonous spp. And Aeromonas spp. And the heavy metals. The composite
showed a positive result in the elimination and removal of water contaminant.