Abstract:
Aim: to evaluate the single and combined effect of Grape Seed Extract
and nanoHydroxyApatite on surface topography and calcium content of
bleached enamel using Scanning electron microscope and EnergyDispersive X-ray Analysis.
Materials and methods: 35 maxillary first premolars were assigned to
five groups, seven premolars each. According to the procedure done:
Group I (control group), group II (bleached), group III (bleached
treated with grape seed extract), group IV (bleached treated with
nanohydroxyapatite), and group V (bleached treated with both
materials). After that teeth of each group were left in artificial saliva for
24hrs.
Results: Both groups III & IV revealed less pronounced perikymata
ridges and grooves and precipitations scattered on rodless enamel or
caused partial occlusion of enamel rod ends. Group V enamel rod ends
were small in diameter so appear as pitting on the surface of the
enamel. Another variant presented almost homogenous deposition of
the precipitates covered most of the enamel surface leading to almost
occluded enamel rod ends that could hardly be identified.
Energy-Dispersive X-ray Analysis showed that Ca mean weight
percentage of group V were similar to group I with no statistical
significant difference while it was significantly higher than group II
while groups III & IV Ca mean weight percentages were significantly
lower than the control group (group I) while there was no significant
difference when compared to the bleached group (group II).
Conclusion: it was found that combining Grape Seed Extract and
nanoHydroxyApatite encouraged remineralization after tooth
demineralization by a bleaching agent.
