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| dc.contributor.author | H. A. Hussein, Ebtissam | |
| dc.contributor.author | S. Adawy, Sami | |
| dc.contributor.author | E. M. E. Ismail, Samer | |
| dc.contributor.author | A. El-Itriby, Hanaiya | |
| dc.date.accessioned | 2020-03-05T09:18:45Z | |
| dc.date.available | 2020-03-05T09:18:45Z | |
| dc.date.issued | 2005 | |
| dc.identifier.citation | Adawy, S.S., Hussein, Ebtissam H.A., ElKhishin, Dina, Moharam, H. and ElItriby, Hanayia A. (2002). Genetic variability studies and molecular fingerprinting of some Egyptian date palm (Phoenix dactylifera L.) cultivars: II. RAPD and ISSR profiling. Arab J. Biotech., 5(2): 225-236. Adawy, S.S., Hussein, Ebtissam H.A., Saker, M.M. and El-Itriby, Hanaiya A. (2004). Intra- and Inter-varietal variation of Upper Egypt date palm cultivars (Phoenix dactylifera L.): I. As revealed by RAPD and ISSR markers. Proceed. Int. Conf. Genet. Eng. & Appl., Sharm El-Sheikh, South Sinai, Egypt (April, 8-11, 2004): 165-179. Ben Saleh, M. and El-Helaly, R. (2003). Using ISSR to investigate the similarities and dissimilarities between some Tunisian date palm (Phoenix dactylifera L.) cultivars. (in Arabic and abstract in English). 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(in Arabic and abstract in English). International Conference on Date Palm, Kingdom of Saudia Arabia, Ministry of Higher Education, King Saud University, Qaseem branch, College of Agriculture and Veterinary Medicine (16-19 September 2003). Ulanovsky, S., Gogrcena, Y., Martίnez de Toda, F. and Ortiz, J.M. (2002). Use of molecular markers in detection of synonymies and homonymies in grapevines (Vitis vinifera L.). Scentia Horticulturae, 92: 241-254. Williams, J.G.K., Kublick, A.R., Livak, K.J., Rafalski, J.A. and Tingey, S.V. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research, 18: 6531- 6535. | en_US |
| dc.identifier.uri | https://t.ly/ONZ9r | |
| dc.description | MSA Google Scholar | en_US |
| dc.description.abstract | The genetic variability and relationships among 14 date palm (Phoenix dactylifera L.) accessions representing six Egyptian cultivars were assayed using 27 RAPD and 10 ISSR primers. The level of polymorphism among the 14 accessions as revealed by RAPD and ISSR was 25.2% and 28.6%, respectively. These low levels of polymorphism reflect the narrow genetic background of these accessions. The genetic relationships among the 14 accessions were estimated in terms of similarity using Dice coefficients. The genetic similarity ranged from 96.1% to 99.5% and from 91.2% to 100% for RAPD and ISSR, respectively. The inter-cultivar relationships among the six date palm cultivars based on RAPD and ISSR revealed the highest genetic similarity between the cultivar Bertmoda and each of the cultivars Malkaby and Sakkoty. The RAPD and ISSR based dendrograms clustered the accessions belonging to each of the 3 cultivars Fraihy, Siwi and Gandila in separate groups. However, the reshuffling in the position of some of the accessions belonging to the other cultivars in the different dendrograms revealed that they share common gnenetic background. Cultivar-specific DNA markers characterized different genotypes and therefore, were used to generate unique fingerprint for each genotype. The RAPD and ISSR revealed 17 and 5 cultivar unique DNA markers, characterizing 4 and 5 cultivars, respectively. Moreover, each of the RAPD and ISSR was successful in identifying accession-specific markers characterizing five accessions. | en_US |
| dc.description.sponsorship | Arab Journal of Biotechnology | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | Arab Journal of Biotechnology | en_US |
| dc.relation.ispartofseries | Arab Journal of Biotechnology;Volume: 8 Issue: 1 Pages: 83-98 | |
| dc.subject | University of Date palm, DNA markers, RAPD, ISSR, Unique markers, Cluster analysis | en_US |
| dc.title | Molecular characterization of some Egyptian date palm germplasm using RAPD and ISSR markers | en_US |
| dc.type | Article | en_US |
| dc.Affiliation | October University for modern sciences and Arts (MSA) |
