Abstract:
Interleukin-10 is involved in carcinogenesis by supporting tumor escape from the immune response. The aim of this study
was to assess the single nucleotide polymorphisms, −1082A/G, −819T/C and −592A/C, in interleukin-10 gene promoter
in inflammatory breast cancer compared to non–inflammatory breast cancer and association of these polymorphisms
with interleukin-10 gene expression. We enrolled 105 breast cancer tissue (72 non–inflammatory breast cancer and
33 inflammatory breast cancer) patients and we determined the three studied single nucleotide polymorphisms in all
samples by polymerase chain reaction restriction fragment length polymorphism and investigated their association with
the disease and with various prognostic factors. In addition, we assessed the expression of interleukin-10 gene by realtime quantitative reverse transcription polymerase chain reaction and the correlation between studied single nucleotide
polymorphisms and interleukin-10 messenger RNA expression. We found co-dominant effect as the best inheritance
model (in the three studied single nucleotide polymorphisms in non–inflammatory breast cancer and inflammatory breast
cancer samples), and we didn’t identify any association between single nucleotide polymorphisms genotypes and breast
cancer prognostic factors. However, GCC haplotype was found highly associated with inflammatory breast cancer risk
(p<0.001, odds ratio=43.05). Moreover, the expression of interleukin-10 messenger RNA was significantly higher
(p<0.001) by 5.28-fold and 8.95-fold than non–inflammatory breast cancer and healthy control, respectively, where
GCC haplotype significantly increased interleukin-10 gene expression (r=0.9, p<0.001).
