Correlation between a single nucleotide polymorphism (G/T at nt –88) in the Mx1 gene promoter and the response to interferon therapy for hepatitis C virus in Egyptian patients
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Correlation between a single nucleotide polymorphism (G/T at nt –88) in the Mx1 gene promoter and the response to interferon therapy for hepatitis C virus in Egyptian patients
S. Mohamed, Mervat; F. Sabet, Salwa; M. Moustafa, Mohamed; S. Salama, Mohamed
Series Info:African Journal of Biotechnology;Vol. 10(62), pp. 13376-13382,
Type:Article
Keywords:Hepatitis C virus (HCV)
,
interferon (IFN)
,
myxovirus resistance protein (Mx1 protein)
,
myxovirus resistance gene-1 (Mx1 gene)
,
single nucleotide polymorphism (SNP).
Abstract:
Interferon used in the treatment of hepatitis C virus (HCV) patients stimulates the expression of a
number of host genes encoding enzymes with antiviral activities, including myxovirus resistance gene1 (Mx1). Mx1 gene was found to have a single nucleotide polymorphism (SNP) at position -88 in the
promotor region that affect the expression of Mx 1 protein and was suggested to be associated with the
response of HCV. In this study, we assessed the relation between the SNP in the Mx1 gene and the
responsiveness of Egyptian HCV patients to pegylated interferon and ribavirin treatment along with
other host-related and virus-related predictors of treatment outcome. We genotyped the biallelic G/T
SNP in the promoter region of Mx1 gene at position -88 from the transcription start site by restriction
fragment length polymorphism (RFLP) in 42 interferon treatment-naïve Egyptian patients that were
treated with pegylated interferon and ribavirin. We found that Mx1 nt-88 SNP is not significantly
correlated to achieving sustained virological response (SVR) after pegylated interferon alpha and
ribavirin combined treatment. We conclude that Mx1 gene polymorphism at codon nt-88 cannot be
considered as biological marker to potentially identify responders and non-responders of HCV patients
to achieve a sustained virological response to treatment with interferon (IFN) in combination with
ribavirin.