Identification of drought-inducible genes and differentially expressed sequence tags in barley

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dc.contributor.author Diab, Ayman A
dc.contributor.author Teulat-Merah, Béatrice
dc.contributor.author This, Dominique
dc.contributor.author Z Ozturk, Neslihan
dc.contributor.author Benscher, David
dc.contributor.author E Sorrells, Mark
dc.date.accessioned 2020-01-21T11:06:39Z
dc.date.available 2020-01-21T11:06:39Z
dc.date.issued 2004
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dc.identifier.other https://doi.org/10.1007/s00122-004-1755-0
dc.identifier.uri https://qrgo.page.link/BYo85
dc.description MSA Google Scholar en_US
dc.description.abstract Drought limits cereal yields in several regions of the world and plant water status plays an important role in tolerance to drought. To investigate and understand the genetic and physiological basis of drought tolerance in barley, differentially expressed sequence tags (dESTs) and candidate genes for the drought response were mapped in a population of 167 F8 recombinant inbred lines derived from a cross between “Tadmor” (drought tolerant) and “Er/Apm” (adapted only to specific dry environments). One hundred sequenced probes from two cDNA libraries previously constructed from drought-stressed barley (Hordeum vulgare L., var. Tokak) plants and 12 candidate genes were surveyed for polymorphism, and 33 loci were added to a previously published map. Composite interval mapping was used to identify quantitative trait loci (QTL) associated with drought tolerance including leaf relative water content, leaf osmotic potential, osmotic potential at full turgor, water-soluble carbohydrate concentration, osmotic adjustment, and carbon isotope discrimination. A total of 68 QTLs with a limit of detection score ≥2.5 were detected for the traits evaluated under two water treatments and the two traits calculated from both treatments. The number of QTLs identified for each trait varied from one to 12, indicating that the genome contains multiple genes affecting different traits. Two candidate genes and ten differentially expressed sequences were associated with QTLs for drought tolerance traits. en_US
dc.language.iso en en_US
dc.publisher Springer-Verlag en_US
dc.relation.ispartofseries Theoretical and Applied Genetics;109, pages1417–1425
dc.subject Quantitative Trait Locus en_US
dc.subject Drought Stress en_US
dc.subject Drought Tolerance en_US
dc.subject Relative Water Content en_US
dc.subject Osmotic Adjustment en_US
dc.title Identification of drought-inducible genes and differentially expressed sequence tags in barley en_US
dc.type Article en_US
dc.identifier.doi https://doi.org/10.1007/s00122-004-1755-0
dc.Affiliation October University for modern sciences and Arts (MSA)


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