The potential role of Azadirachta indica treatment on cisplatin-induced hepatotoxicity and oxidative stress in female rats

Dkhil M.A.; Al-Quraishy S.; Aref A.M.; Othman M.S.; El-Deib K.M.; Abdel Moneim A.E.

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The potential role of Azadirachta indica treatment on cisplatin-induced hepatotoxicity and oxidative stress in female rats

Dkhil M.A.; Al-Quraishy S.; Aref A.M.; Othman M.S.; El-Deib K.M.; Abdel Moneim A.E.

Full Text link: https://t.ly/DXXAY

Date issued: 2013

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Doi: https://doi.org/10.1155/2013/741817 PubMed ID : 24369490

Series Info: Oxidative Medicine and Cellular Longevity

Type: Article

Keywords: antioxidant , caspase 3 , caspase 9 , catalase , cisplatin , immunoglobulin enhancer binding protein , messenger RNA , plant extract , protective agent , protein Bax , superoxide dismutase , animal , article , Azadirachta indica , chemistry , drug effect , enzymology , female , gene expression regulation , genetics , lipid peroxidation , liver , metabolism , oxidative stress , pathology , phytotherapy , plant leaf , rat , toxic hepatitis , Wistar rat , Animals , Antioxidants , Azadirachta , bcl-2-Associated X Protein , Caspase 3 , Caspase 9 , Catalase , Cisplatin , Drug-Induced Liver Injury , Female , Gene Expression Regulation , Lipid Peroxidation , Liver , NF-kappa B , Oxidative Stress , Phytotherapy , Plant Extracts , Plant Leaves , Protective Agents , Rats , Rats, Wistar , RNA, Messenger , Superoxide Dismutase

Abstract:

Azadirachta indica A. Juss. (neem, family: Meliaceae) is perhaps the most commonly used traditional medicinal plant of India. In this study we investigated the protective effect of methanolic neem leaves extract (MNLE; 500 mg/Kg bwt) on rats treated with cisplatin (CDDP)-induced hepatotoxicity. Adult rats were randomly divided into four groups. CDDP was given to rats by intraperitoneal injection, while MNLE was given by oral gavage for 5 days after the CDDP injection. The injury and oxidative stress caused by CDDP on the liver and the effect of MNLE were evaluated by measuring (a) histological changes, (b) tissue biochemical oxidant and antioxidant parameters, and (c) investigating apoptosis markers immunohistochemically and by real time PCR. After treatment with MNLE, the histological damage and apoptosis induction caused by cisplatin were improved. Malondialdehyde and nitric oxide were significantly decreased; the antioxidant system, namely, glutathione content, glutathione-S-transferase, glutathione peroxidase, catalase, and superoxide dismutase activities were significantly elevated. In conclusion, MNLE may have a potential role when combined with cisplatin in chemotherapy to alleviate cisplatin-induced damage and oxidative stress in liver. 2013 Mohamed A. Dkhil et al.