Effect of Physalis peruviana L. on cadmium-induced testicular toxicity in rats
Othman M.S.; Nada A.; Zaki H.S.; Abdel Moneim A.E.
Date issued:
2014
Publisher:
MDPI AG
,
MDPI AG
,
Humana Press Inc.
Series Info:
Biological Trace Element Research
159
Type:
Article
Keywords:
October University for Modern Sciences and Arts
,
University for Modern Sciences and Arts
,
MSA University
,
جامعة أكتوبر للعلوم الحديثة والآداب
,
Apoptosis
,
Cadmium
,
Oxidative stress
,
Physalis peruviana L
,
Testes
,
cadmium
,
cadmium chloride
,
caspase 3
,
catalase
,
glutathione
,
glutathione peroxidase
,
glutathione reductase
,
malonaldehyde
,
nitric oxide
,
Physalis peruviana extract
,
plant extract
,
protective agent
,
superoxide dismutase
,
testosterone
,
unclassified drug
,
cadmium
,
caspase 3
,
glutathione
,
malonaldehyde
,
plant extract
,
adult
,
animal experiment
,
animal model
,
animal tissue
,
apoptosis
,
article
,
cadmium induced testicular toxicity
,
controlled study
,
down regulation
,
enzyme activity
,
fruit
,
germ cell
,
histopathology
,
immunohistochemistry
,
lipid peroxidation
,
male
,
nonhuman
,
oxidative stress
,
Physalis
,
physalis peruviana
,
protein expression
,
rat
,
spermatogenesis
,
steroidogenesis
,
testis disease
,
testis injury
,
testis weight
,
testosterone blood level
,
weight change
,
animal
,
chemistry
,
drug effects
,
metabolism
,
testis
,
toxicity
,
Physalis
,
Physalis peruviana
,
Rattus
,
Solanaceae
,
Animals
,
Apoptosis
,
Cadmium
,
Caspase 3
,
Glutathione
,
Male
,
Malondialdehyde
,
Oxidative Stress
,
Physalis
,
Plant Extracts
,
Rats
,
Testis
Abstract:
Cadmium (Cd) stimulates the production of reactive oxygen species and causes tissue damage. We investigated here the protective effect of Physalis peruviana L. (family Solanaceae) against cadmium-induced testes toxicity in rats. Twenty-eight Wistar albino rats were used. They were divided into four groups (n=7). Group 1 was used as control. Group 2 was intraperitoneally injected with 6.5 mg/kg body weight (bwt) of cadmium chloride for 5 days. Group 3 was orally treated with 200 mg/kg bwt of methanolic extract of physalis (MEPh). Group 4 was pretreated with MEPh before cadmium for 5 days. Changes in body and testes weights were determined. Oxidative stress markers, antioxidant enzymes, and testosterone level were measured. Histopathological changes of testes were examined, and the immunohistochemical staining for the proapoptotic (caspase-3) protein was performed. The injection of cadmium caused a significant decrease in body weight, while a significant increase in testes weight and testes weight index was observed. Pretreatment with MEPh was associated with significant reduction in the toxic effects of Cd as shown by reduced testicular levels of malondialdehyde, nitric oxide, and caspase-3 expression and increased glutathione content, and the activities of superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase, and testosterone were also increased. Testicular histopathology showed that Cd produced an extensive germ cell apoptosis, and the pretreatment of MEPh in Cd-treated rats significantly reduced Cd-induced testicular damage. On the basis of the above results, it can be hypothesized that P. peruviana L. has a protective effect against cadmium-induced testicular oxidative stress and apoptosis in the rat. � 2014 Springer Science+Business Media.
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