Antigen content as The Min factor In Avian Influence Vaccine Efficiency

Abstract

Breast cancer is the leading causes of cancer-related death among women. The vast majority of breast cancers are carcinomas that originate from cells lining the milk-forming ducts of the mammary gland. The human cytomegalovirus (HCMV) is a multifaceted highly host specific betaherpesvirus that is regarded as asymptomatic or mildly pathogenic virus in immunocompetent host. HCMV may cause acute and chronic complications in immunocompromised individual. HCMV antigens and DNA have been detected in tissue biopsies of breast cancers and sentinel lymph nodes and elevation in serum HCMV IgG antibody levels have been reported to precede the development of breast cancer in some women. The aim of the study is to evaluate the association of human cytomegalovirus (HCMV) incidence and lymph node metastases in Egyptian breast cancer patients. The project was carries out through testing of enrolled breast cancer patients for presence or absence of HCMV IgG antibody in their blood samples. Moreover, nested PCR was done on DNA extracts from patients’ lymph node tissue targeting the HCMV immediate early (IE) gene. Finally, correlation between incidence of HCMV DNA and lymph node metastasis in breast cancer patients was assessed. Outcome of the study displays that there is a statistical significance between HCMV-DNA and lymph node metastasis.