Dual approach for the colorimetric determination of unamplified microRNAs by using citrate capped gold nanoparticles

Nossier A.I.; Abdelzaher H.; Matboli M.; Eissa S.

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dc.contributor.author	Nossier A.I.
dc.contributor.author	Abdelzaher H.
dc.contributor.author	Matboli M.
dc.contributor.author	Eissa S.
dc.contributor.other	Biochemistry Department
dc.contributor.other	Faculty of Pharmacy
dc.contributor.other	Misr University for Science and Technology (MUST)
dc.contributor.other	6th October City
dc.contributor.other	Giza
dc.contributor.other	Egypt; Faculty of Biotechnology
dc.contributor.other	October University for Modern Sciences & Arts
dc.contributor.other	6th October City
dc.contributor.other	Cairo
dc.contributor.other	Egypt; Oncology Diagnostic Unit
dc.contributor.other	Medical Biochemistry & Molecular Biology Department
dc.contributor.other	Faculty of Medicine
dc.contributor.other	Ain Shams University
dc.contributor.other	Cairo
dc.contributor.other	Egypt; Faculty of Medicine Ain Shams Research Institute (MASRI)
dc.contributor.other	Cairo
dc.contributor.other	Egypt
dc.date.accessioned	2020-01-09T20:40:56Z
dc.date.available	2020-01-09T20:40:56Z
dc.date.issued	2018
dc.identifier.issn	263672
dc.identifier.other	https://doi.org/10.1007/s00604-018-2767-9
dc.identifier.other	PubMedID29594755
dc.identifier.uri	https://t.ly/rxxeW
dc.description	Scopus
dc.description.abstract	The authors describe a method for the colorimetric determination of unamplified microRNA. It is based on the use of citrate-capped gold nanoparticles (AuNPs) and, alternatively, a microRNA-probe hybrid or a magnetically extracted microRNA that serve as stabilizers against the salt-induced aggregation of AuNPs. The absorbance ratios A525/A625 of the reacted AuNP solutions were used to quantify the amount of microRNA. The assay works in the range of 5�25�pmol microRNA. The lower limit of detection (LOD) is 10�pmol. The performance of the method was tested by detection of microRNA-210-3p in totally extracted urinary microRNA from normal, benign, and bladder cancer subjects. The sensitivity and specificity for qualitative detection of urinary microRNA-210-3p using the assay are 74% and 88% respectively, which is consistent with real time PCR based assays. The assay was applied to the determination of specific microRNA by using its specific oligo targeter or following magnetic isolation of the desired microRNA. The method is simple, cost-efficient, has a short turn-around time and requires minimal equipment and personnel. � 2018, Springer-Verlag GmbH Austria, part of Springer Nature.	en_US
dc.language.iso	English	en_US
dc.publisher	Springer-Verlag Wien	en_US
dc.relation.ispartofseries	Microchimica Acta
dc.relation.ispartofseries	185
dc.subject	AuNPs	en_US
dc.subject	Bladder cancer	en_US
dc.subject	Magnetic nanoparticles	en_US
dc.subject	MicroRNA detection	en_US
dc.subject	MicroRNA-210-3p	en_US
dc.subject	Oligonucleotide adsorption	en_US
dc.subject	Oligotargeter	en_US
dc.subject	Salt-induced aggregation	en_US
dc.subject	Streptavidin	en_US
dc.subject	citric acid	en_US
dc.subject	gold	en_US
dc.subject	metal nanoparticle	en_US
dc.subject	microRNA	en_US
dc.subject	MIRN210 microRNA, human	en_US
dc.subject	chemistry	en_US
dc.subject	colorimetry	en_US
dc.subject	cost benefit analysis	en_US
dc.subject	economics	en_US
dc.subject	human	en_US
dc.subject	isolation and purification	en_US
dc.subject	procedures	en_US
dc.subject	urine	en_US
dc.subject	Citric Acid	en_US
dc.subject	Colorimetry	en_US
dc.subject	Cost-Benefit Analysis	en_US
dc.subject	Gold	en_US
dc.subject	Humans	en_US
dc.subject	Metal Nanoparticles	en_US
dc.subject	MicroRNAs	en_US
dc.title	Dual approach for the colorimetric determination of unamplified microRNAs by using citrate capped gold nanoparticles	en_US
dc.type	Article	en_US
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dcterms.source	Scopus
dc.identifier.doi	https://doi.org/10.1007/s00604-018-2767-9
dc.identifier.doi	PubMedID29594755
dc.Affiliation	October University for modern sciences and Arts (MSA)