Series Info:European Annals of Allergy and Clinical Immunology 51
Type:Article
Keywords:October University for Modern Sciences and Arts
,
جامعة أكتوبر للعلوم الحديثة والآداب
,
University of Modern Sciences and Arts
,
MSA University
,
Autoantibodies
,
Biomarkers
,
Celiac disease
,
Gluten free diet
,
MicroRNA
,
immunoglobulin A antibody
,
microRNA 21
,
microRNA 31
,
protein glutamine gamma glutamyltransferase
,
adolescent
,
Article
,
celiac disease
,
child
,
childhood disease
,
clinical article
,
controlled study
,
down regulation
,
female
,
gene expression
,
gluten free diet
,
human
,
male
,
reverse transcription polymerase chain reaction
,
upregulation
Abstract:
Celiac disease is an enteropathy induced by ingestion of gluten triggering an immune response in genetically predisposed individuals. MiRNAs are small non-coding RNAs that have a role as regulators of gene expression at the post transcriptional level. The aim of this study is to evaluate the possibility of using circulating miRNAs as non-invasive biomarkers in pediatric patients with celiac disease. In addition, we examine the effect of a gluten-free diet on the expression of these miRNAs in serum of CD patients. The expression pattern of miR-21 and miR-31 was estimated in serum of 25 untreated CD patients (recently diagnosed), 25 treated CD patients (on gluten-free diet) and 20 healthy controls using qRT-PCR. Our results demonstrated the significant up-regulation of microRNA-21 in the untreated celiac patients in comparison with the treated group and healthy controls. Moreover, miR-31 expression was significantly under-expressed in the untreated celiac patients in comparison with the treated group and healthy controls. Furthermore, the results showed that miR-21 expression level was significantly positively correlated with the tTG IgA auto-antibodies. In conclusion, circulating miRNA-21 and miRNA-31 could serve as potential non-invasive biomarkers for pediatric CD patients. � 2019, EDRA S.p.A. All rights reserved.