Abstract:
Skin aging is a complex of biological process which affected by constant exposure to ultraviolet
(UV) irradiation with subsequent generation of reactive oxygen species leading to collagen
deficiency and eventually skin wrinkling. Different techniques were reported for treatment;
however the application of anti-aging creams is still the best choice among various treatment
methods to their nourishing effect. Apart from that herbal formulation has widely demanded in
the world market for its safety. In the present study lipid nanoparticles (LNPs) are used in the
formulation of anti-aging Citrus Sinensis (CS) cream due to its safety and biocompatibility of its
lipid components. Eight different formulations of CS-LNPs were prepared and optimized using 2
3 full factorial designs. Three factors were evaluated drug concentration (X1), homogenization
speed (X2) and nanoparticle type (X3). Different parameters were measured including; zeta
potential (ZP), polydispersity index (PDI), particle size, entrapment efficiency (EE %) and skin
occlusion test. Morphological study by using scanning electron microscopes (SEM) confirms the
nanometric size of the prepared formulations with spherical and smooth surface. It was suggested
that the optimized formulation (F7) was found to have (245μm) particle size, (91.065%)
Entrapment Efficiency, and (91.385) Occlusive effect. In vitro permeation study of the optimized
formulation through synthetic membrane showed prolonged release of medication within 24 hrs.
Finally, it can be concluded that the prepared anti-aging CS/NLC cream was safe, convenient,
and effective carrier to deliver the herbal medication it also showed better penetration into the
skin with a significant anti-aging effect thus it will be a promising product in skin care
cosmetics.
