Abstract:
The use of nanotechnology in cancer therapy is based on the usage of molecules that
can affect cancerous cells as well as mitochondrial membrane in order to induce cancer cell
programmed death. This method has shown overwhelming effects; hence the target of this
project is to evaluate the role of gold nanoparticles’ anti-cancer potential under the effect of
electroporation, in addition to the effect of ultra low electromagnetic field (ULEMF)
frequencies on HCT 116 colon cancer cell line. It was noticed that this method of using
ULEMF displayed a higher potential of anti-cancer activity than the use of gold nanoparticles
used single handed (engulfed endocytosis). At the same time cell apoptosis that is
accompanied with high percentage of cell arrest in case of treatment with electroporation,
gold nanoparticles as well as electromagnetic field of 50 mT for 30 mins showed the best
results in comparison with other treatments. Also, it has been signified that the usage of these
treatment combinations resulted in a highly elevated gene expression of pro-apoptotic genes
(P53, Bax) and down regulation of anti-apoptotic gene (Bcl-2). It was also noticed that an
arrest of cells occurred at the G2/M phase compared to other treatment regimes. Data
recorded revealed that the use of gold nanoparticles, electroporation and electromagnetic field
in a dose dependent manner can increase apoptosis and necrosis [200v]- [25 mT— 50 mT]. It
can be suggested that the use of electromagnetic field as well as, gold nanoparticles and
electroporation can be a promising tool for cancer management.
