Abstract:
Infertility is the inability to conceive offsprings after 12 month of frequent
mating. It is caused by hormonal, physiological and environmental misbalancing.
Moreover, Premature ovarian failure, endometriosis and polycystic ovarian
syndrome causes female infertility which will be discussed in this study regarding
genes associated with those diseases. Therefore, the enrolled project is about
comparative genomic study of post natal blood samples of females suffering from
infertility using Microarray CGH. Blood samples were collected from the patients
by the host institution, then the samples were prepared in the lab using Thermo
Scientific GeneJET Genomic DNA Purification Kit #K0722 and Agilent
Oligonucleotide Array-Based CGH for Genomic DNA Analysis protocols. The
interpretation of results were done using Agilent feature extraction and
cytogenomics software. Patients‟ results were interpreted and analysed showing
deletion and amplification in genes located on chromosomes associated with
infertility. These genes could be used and targeted in further studies and
researches in manufacturing drugs.
