dc.contributor.author |
Othman, Abdelhameed M. |
|
dc.contributor.author |
El-Houseini, M. E. |
|
dc.contributor.author |
El-Sofy, Mohamed S. |
|
dc.contributor.author |
Aboul-Enein, Hassan Y. |
|
dc.date.accessioned |
2019-11-27T08:57:08Z |
|
dc.date.available |
2019-11-27T08:57:08Z |
|
dc.date.issued |
2011 |
|
dc.identifier.citation |
Cited References in Web of Science Core Collection: 36 |
en_US |
dc.identifier.issn |
1618-2642 |
|
dc.identifier.other |
https://doi.org/10.1007/s00216-011-4774-0 |
|
dc.identifier.uri |
https://link.springer.com/article/10.1007%2Fs00216-011-4774-0 |
|
dc.description |
Accession Number: WOS:000289297000022 |
en_US |
dc.description.abstract |
The activity of the alpha-l-fucosidase (AFU) enzyme represents an excellent test for diagnosis of hepatocellular carcinoma (HCC) and fucosidosis recognized in inborn disorder of metabolism and increases the sensitivity of detection to 95.5% in patients with HCC. Therefore, the determination of the activity of AFU enzyme is very important and can be used as a screening tool for the early diagnosis of tumors for HCC patients. A simple, accurate, and sensitive potentiometric method was developed for measuring the activity of AFU. The method was based upon measuring the concentration of 2-chloro-4-nitrophenol (2-chloro-4-NP) using a 2-chloro-4-NP-rhodamine B ion pair in a PVC membrane sensor. The electrode shows a linear, reproducible, and stable potentiometric response with an anionic Nernstian slope of -51.13 +/- 0.6 mV/decade over a wide range of concentrations 10(-5)-10(-2) M and a detection limit of 1.0 x 10(-6) M of 2-chloro-4-NP. The membrane exhibits a fast response time of 30 s, over a pH range of 4.0-6.5. The selectivity coefficients indicate excellent selectivity for 2-chloro-4-NP over a number of interfering species, e.g., chloride, nitrate, sulfate, chromate urea, albumin, glucose, uric acid, and total protein. The prepared sensor has been used successfully for the determination of 2-chloro-4-NP produced from the hydrolysis of 2-chloro-4-NP-alpha-l-fucopyranoside substrate. It was also applied for the determination alpha-l-fucosidase enzyme of 33 serum samples of healthy subjects and patients. The average recoveries +/- RSD for the healthy subjects, cirrhosis of chronic hepatitis C and B, and HCC serum samples were 102.6 +/- 1.01%, 101.5 +/- 0.95%, and 100.1 +/- 1.1%, respectively. The results obtained are in good agreement with those obtained by standard methods. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
SPRINGER HEIDELBERG |
en_US |
dc.relation.ispartofseries |
ANALYTICAL AND BIOANALYTICAL CHEMISTRY;Volume: 400 Issue: 3 Pages: 787-795 |
|
dc.relation.uri |
https://cutt.ly/LeMgP73 |
|
dc.subject |
University for Rhodamine B (Rd) |
en_US |
dc.subject |
Potentiometry |
en_US |
dc.subject |
PVC membrane sensor |
en_US |
dc.subject |
alpha-L-Fucosidase enzyme |
en_US |
dc.subject |
HEPATOCELLULAR-CARCINOMA |
en_US |
dc.subject |
MARKERS |
en_US |
dc.subject |
ASSAY |
en_US |
dc.title |
Potentiometric determination of alpha-l-fucosidase enzyme by using 2-chloro-4-nitrophenol-rhodamine B ion pair chemical recognition in PVC membrane sensor |
en_US |
dc.type |
Article |
en_US |
dc.identifier.doi |
https://doi.org/10.1007/s00216-011-4774-0 |
|
dc.Affiliation |
October University for modern sciences and Arts (MSA) |
|