Mohamed, AhmedReda, AsimTarek, ManarYasser, Sara2019-10-132019-10-132019Copyright © 2019 MSA University. All Rights Reserved.https://t.ly/DZdV3Skin aging is a complex of biological process which affected by constant exposure to ultraviolet (UV) irradiation with subsequent generation of reactive oxygen species leading to collagen deficiency and eventually skin wrinkling. Different techniques were reported for treatment; however the application of anti-aging creams is still the best choice among various treatment methods to their nourishing effect. Apart from that herbal formulation has widely demanded in the world market for its safety. In the present study lipid nanoparticles (LNPs) are used in the formulation of anti-aging Citrus Sinensis (CS) cream due to its safety and biocompatibility of its lipid components. Eight different formulations of CS-LNPs were prepared and optimized using 2 3 full factorial designs. Three factors were evaluated drug concentration (X1), homogenization speed (X2) and nanoparticle type (X3). Different parameters were measured including; zeta potential (ZP), polydispersity index (PDI), particle size, entrapment efficiency (EE %) and skin occlusion test. Morphological study by using scanning electron microscopes (SEM) confirms the nanometric size of the prepared formulations with spherical and smooth surface. It was suggested that the optimized formulation (F7) was found to have (245μm) particle size, (91.065%) Entrapment Efficiency, and (91.385) Occlusive effect. In vitro permeation study of the optimized formulation through synthetic membrane showed prolonged release of medication within 24 hrs. Finally, it can be concluded that the prepared anti-aging CS/NLC cream was safe, convenient, and effective carrier to deliver the herbal medication it also showed better penetration into the skin with a significant anti-aging effect thus it will be a promising product in skin care cosmetics.enOctober University for Modern Sciences and ArtsUniversity of Modern Sciences and ArtsMSA Universityجامعة أكتوبر للعلوم الحديثة والآدابPharmaceuticsNanoformulationOther