Aborehab N.M.El Bishbishy M.H.Waly N.E.Faculty of PharmacyMSA UniversityDepartment of BiochemistryGiza11787Egypt; Faculty of PharmacyMSA UniversityDepartment of PharmacognosyGiza11787Egypt; Faculty of MedicineHelwan UniversityDepartment of PhysiologyHelwan11787Egypt2020-01-092020-01-09201614726882https://doi.org/10.1186/s12906-016-1203-0PubMed ID 27430475https://t.ly/yK3zDScopusBackground: Resistin is an adipocyte hormone that regulates glucose metabolism. Elevated levels of resistin may cause insulin resistance. This may link obesity, and increased fat mass to type II diabetes and insulin resistance. We hypothesized that treatment with tomato and broccoli extracts regulates glucose homeostasis via modulation of resistin levels in high fat diet-induced obesity rats (HFD). Methods: Forty-eight male albino rats were divided into 8 groups as follows: control, HFD, stop fat diet (SD), Tomato 200 mg/kg (T200), Tomato 400 mg/kg (T400), Broccoli 200 mg/kg (B200), Broccoli 400 mg/kg (B400), and Chromax (CX). Treatment continued for 1 month. Serum levels of resistin, leptin, adiponectin, glucose and insulin were measured using ELISA and spectrophotometry. Results: Serum levels of resistin were significantly reduced in the T 200, T 400, B 200, B 400 and CX groups to: 4.13 � 0.22 ng/ml, 1.51 � 0.04 ng/ml, 4.13 � 0.22 ng/ml, 2.32 � 0.15 ng/ml and 1.37 � 0.03 ng/ml, respectively, compared to HFD group and SD group (P value < 0.0001). Non-significant differences were found between T 400, B 400 and CX groups. Serum levels of leptin were significantly reduced in the T 400 (22.7 � 0.84 pg/ml) group compared to the B 400 (41 � 2.45 Pg/ml) and CX groups (45.7 � 2.91 Pg/ml), P value < 0.001. Serum levels of adiponectin were significantly increased in the T 400 group (131 � 3.84 pg/ml) compared to the CX group (112 � 4.77 pg/ml), P value < 0.01. Conclusions: Our results demonstrate that tomato and broccoli extract treatment regulates glucose homeostasis via reduction of serum resistin and may be a useful non-pharmacological therapy for obesity. � 2016 The Author(s).EnglishOctober University for Modern Sciences and Artsجامعة أكتوبر للعلوم الحديثة والآدابUniversity of Modern Sciences and ArtsMSA UniversityBroccoliGlucose homeostasisObesityResistinTomatoadiponectinantioxidantbroccoli extractchromium picolinateglucoseinsulinleptinplant extractresistintomato extractunclassified drugadiponectinglucose blood levelleptinplant extractresistinanimal experimentanimal modelanimal tissueantioxidant activityArticlebroccolicontrolled studydiet induced obesityenzyme linked immunosorbent assayglucose blood levelinsulin blood levellimit of quantitationlipid dietmalenonhumanprotein blood levelratspectrophotometrytomatoanimalbloodBrassicachemistrydrug effectsmetabolismobesitypathophysiologyphysiologytomatoAdiponectinAnimalsBlood GlucoseBrassicaDiet, High-FatLeptinLycopersicon esculentumMaleObesityPlant ExtractsRatsResistinArticlehttps://doi.org/10.1186/s12906-016-1203-0PubMed ID 27430475