Hamed M.M.Abd El-Mobdy M.A.Kamel M.T.Mohamed H.I.Bayoumi A.E.Medicinal Chemistry DepartmentTheodor Bilharz Research InstituteGizaEgypt; Faculty of BiotechnologyOctober University for Modern Sciences and Arts (MSA)Egypt; Biological and Geological Sciences DepartmentFaculty of EducationAin Shams UniversityEgypt2020-01-092020-01-09201918278620https://t.ly/pywKAScopusIdentifying ornamental plants as new natural anticancer sources is always of great effective for the ornamental and horticultural industries. The investigator for Senecio vulgaris were identified by GC-MS to give a total of 14 sesquiterpenoids, represents 98.53% of the total oil, while Pluchea dioscoridis L. shows the standardization of 30 compounds essential oil represents 91.28% of the total oil. Senecio vulgaris essential oil showed a high brine shrimp lethality with LC50 value of 8 ?g/ml followed by Pluchea dioscoridis L with LC50 value of 24 ?g/ml. Following cytotoxic activity was screened by MTT viability test against HePG2 cell line. Inhibitory activity against Hepatocellular carcinoma cells of S. vulgaris and P. dioscoridis L essential oil extract under the experimental conditions exhibited a promising IC50 of 5.63 and 11.50 �g/ml, respectively compared to Doxorubicin as a control. The results demonstrated the potential of the two plants essential oils for cancer treatments; therefore, in the future in vivo experiments are required for further understanding of the effect of these plants on cancer cells to be useful in medicine. � 2019, SILAE (Italo-Latin American Society of Ethnomedicine). All rights reserved.EnglishCytotoxic activityPluchea dioscoridis LSenecio vulgarisVolatile oildoxorubicinessential oilplant extractPluchea dioscoridis extractSenecio vulgaris extractsesquiterpenoidunclassified druganimal experimentArtemiaArticleAsteraceaebiological activitycell viability assaycontrolled studycytotoxicitydrug identificationdrug screeningdrug structureHep-G2 cell linehumanhuman cellIC50LC50lethalitymass fragmentographyMTT assaynonhumanphytochemistryPluchea dioscoridisSenecio vulgarisstandardizationArticle