Ismail, Abdallah RKashtoh, HamdyBetiha, Mohamed AAbu Amr, Salem ABaek, Kwang-HyunEl-Gendy, Nour Sh2022-11-062022-11-062022-11https://doi.org/10.1155/2022/7899996http://repository.msa.edu.eg/xmlui/handle/123456789/5242In this study, evaporation-induced self-assembly was applied to prepare amine-functionalized nano-silica (NH2-Pr-SBA-15). Tat was simply used to immobilize Bacillus stratosphericus PSP8 lipase (E–NH2–Pr-SBA-15), producing a nanobiocatalyst with good stability under vigorous shaking and a maximum lipase activity of 45 ± 2 U/mL. High-resolution X-ray dif- fractometer, Fourier transform infrared spectroscopy, N2 adsorption-desorption, feld-emission scanning electron, and high- resolution transmission electron microscopic analyses proved the successful SBA-15 functionalization and enzyme im- mobilization. Response surface methodology based on a 1/2 fraction-three-levels face center composite design was applied to optimize the biodiesel transesterifcation process. Tis expressed efcient percentage conversion (97.85%) and biodiesel yield (97.01%) under relatively mild operating conditions: 3.12 : 1 methanol to oil ratio, 3.08 wt.% E–NH2–Pr-SBA-15 loading, 48.6°C, 3.19 h at a mixing rate of 495.53 rpm. E–NH2–Pr-SBA-15 proved to have a long lifetime, operational stability, and reusability.en-USValorizationCooking OilBacillusAmine-FunctionalizedSBA-15 NanobiocatalystArticlehttps://doi.org/10.1155/2022/7899996