El-Leithy E.S.Makky A.M.Khattab A.M.Hussein D.G.Department of Pharmaceutics and Industrial PharmacyFaculty of PharmacyOctober University for Modern Sciences and Arts (MSA)CairoEgypt; Department of Pharmaceutics and Industrial PharmacyFaculty of PharmacyHelwan UniversityAin HelwanCairoEgypt; Department of Pharmaceutics and Industrial PharmacyFaculty of PharmacyCairo UniversityCairoEgypt; National Organization for Drug Control and ResearchCairoEgypt2020-01-092020-01-0920183639045https://doi.org/10.1080/03639045.2017.1391836PubMed ID 29096550https://t.ly/y6MVyScopusMSA Google ScholarCoenzyme Q10 (CoQ10) is an insoluble, poorly permeable antioxidant with great biological value which acts as anti-aging and anti-wrinkle agent. To improve its permeability through topical application, the current study aimed at formulating oil/water (o/w) nanoemulsion (NE) as an efficient vehicle for delivering (CoQ10) through the skin barriers. The solubility of (CoQ10) was tested for various oils, surfactants (S), and co-surfactants (CoS). The NE region was determined by constructing pseudoternary phase diagrams. NE formulae containing 1, 2, and 3% w/w drug have been subjected to thermodynamic stability test. The formulae that passed thermodynamic stability tests were characterized by physical properties as pH, viscosity, refractive index, droplet size, zeta-potential, TEM, electroconductivity, in vitro release, and ex vivo permeation. The formula F2 containing 10% w/w isopropyl myristate (oil phase), 60% w/w of Tween 80: Transcutol HP mixture (S/CoSmix) at ratio 2:1, 30% w/w water and 2% w/w drug was evaluated for its anti-wrinkle efficiency using an animal model. The F2 formula showed 11.76 1.1 nm droplet size, 1.4260 0.0016 refractive index, 0.228 PDI, ?14.7 1.23 mv zeta potential, 7.06 0.051 pH, 199.05 0.35 cp viscosity, and the highest percentage of drug release in the selected dissolution media. About 47.21% of the drug was released in phosphate buffer 7.4 containing 5% w/v Labrasol and 5% w/v isopropyl alcohol through 24 h. It also showed the highest drug flux (Jss = 3.164 g/cm2/h), enhancement ratio (Er = 8.32), and permeability coefficient (Kp = 22.14 10?4 cm2/h). CoQ10 NE reduced the skin wrinkles and gave the skin smooth appearance. Our investigation suggests the potential use of NE as a vehicle for enhancing solubility and permeability of CoQ10 and thus improving its anti-wrinkle efficiency. 2017 Informa UK Limited, trading as Taylor & Francis Group.Englishanti-wrinkleCoenzyme Q10nanoemulsionpermeabilityphase diagram2 propanolcremophordiethylene glycol monoethyl etherlabrasolmyristic acid isopropyl esternutraceuticalphosphate buffered salinepolysorbate 80propylene glycolsorbitan laurateubidecarenonewaterwater oil creamnanoparticlesurfactantubidecarenoneubiquinoneanimal experimentanimal modelanimal tissueArticlecontrolled studydrug dosage form comparisondrug efficacydrug penetrationdrug releasedrug solubilityelectric conductivityex vivo studyfemalein vitro studynanoemulsionnanopharmaceuticsnonhumanparticle sizepHratrefraction indexskin permeabilitythermodynamicstransmission electron microscopyviscositywrinklezeta potentialanalogs and derivativesanimalchemistrycutaneous drug administrationcutaneous parametersdrug delivery systemdrug effectsdrug stabilityemulsionmedicinal chemistryphysiologyskin absorptionsolubilitysurface propertyWistar ratAdministration, CutaneousAnimalsChemistry, PharmaceuticalDrug Delivery SystemsDrug LiberationDrug StabilityEmulsionsFemaleHydrogen-Ion ConcentrationNanoparticlesParticle SizeRatsRats, WistarSkin AbsorptionSkin AgingSolubilitySurface PropertiesSurface-Active AgentsUbiquinoneViscosityArticlehttps://doi.org/10.1080/03639045.2017.1391836PubMed ID 29096550