Ninomiya, KensukeIwakiri, JunichiAly, Mahmoud KhamisSakaguchi, YurikoAsai, Shungo KiyoshiSuzuki, TsutomuHirose, Tetsuro2021-07-022021-07-022021https://doi.org/10.15252/embj.2021107976https://qrgo.page.link/fZJuwNuclear stress bodies (nSBs) are nuclear membraneless organelles formed around stress-inducible HSATIII architectural long noncoding RNAs (lncRNAs). nSBs repress splicing of hundreds of introns during thermal stress recovery, which are partly regulated by CLK1 kinase phosphorylation of temperature-dependent Ser/Arg-rich splicing factors (SRSFs). Here, we report a distinct mechanism for this splicing repression through protein sequestration by nSBs. Comprehensive identification of RNA-binding proteins revealed HSATIII association with proteins related to N6 -methyladenosine (m6 A) RNA modification. 11% of the first adenosine in the repetitive HSATIII sequence were m6 A-modified. nSBs sequester the m6 A writer complex to methylate HSATIII, leading to subsequent sequestration of the nuclear m6 A reader, YTHDC1. Sequestration of these factors from the nucleoplasm represses m6 A modification of pre-mRNAs, leading to repression of m6 A-dependent splicing during stress recovery phase. Thus, nSBs serve as a common platform for regulation of temperature-dependent splicing through dual mechanisms employing two distinct ribonucleoprotein modules with partially m6 A-modified architectural lncRNAs.en-USlong noncoding RNAm6 A modificationmolecular spongenuclear stress bodiespre-mRNA splicingTranscription and GenomicsRNA BiologyArticlehttps://doi.org/10.15252/embj.2021107976