Abdalla Khalil, NadiaMohamed Ahmed, EmanOmar Mohamed, KhaledMohammed Nissan, YassinAbo-Bakr Zaitone, Sawsan2020-02-152020-02-1520140968-0896https://doi.org/10.1016/j.bmc.2014.02.042https://cutt.ly/grX4SPoMSA Google ScholarA new series of pyrazolone–pyridazine conjugates 3 and 4a–l were synthesized and characterized by spectroscopic means and elemental analyses. All compounds were tested in vivo for their anti-inflammatory and analgesic properties against diclofenac, as reference compound. The synthesized compounds were also evaluated for their ability to inhibit the production of certain inflammatory cytokines such as TNF-α and IL-6 in serum samples. The ulcerogenic potential of the synthesized compounds was also determined. IC50 values for inhibition of COX-1 and COX-2 enzymes were investigated in vitro for the most active candidates. Molecular docking was performed on the active site of COX-2 to predict their mode of binding to the amino acids. Among the synthesized derivatives, compounds 4c and 4e showed good analgesic and anti-inflammatory activities with lower ulcer index than the reference drugenPyrazolonePyridazineAnalgesicAnti-inflammatoryArticlehttps://doi.org/10.1016/j.bmc.2014.02.042