Elbalkiny H.T.Yehia A.M.Riad S.M.Elsaharty Y.S.Analytical Chemistry DepartmentFaculty of PharmacyOctober University for Modern Sciences and Arts (MSA)6th October City11787Egypt; Analytical Chemistry DepartmentFaculty of PharmacyCairo UniversityKasr-El Aini 13 StreetCairo11562Egypt; Chemistry DepartmentSchool of Pharmacy and Pharmaceutical IndustriesBadr University in CairoBadr CityCairo 11829Egypt2020-01-092020-01-09202013861425https://doi.org/10.1016/j.saa.2019.117903PubMed ID :https://t.ly/veGEbScopusA sensitive accurate spectrofluorimetric technique was developed to detect cefadroxil and cefradine traces in water samples simultaneously, by applying a procedure based on the formation of hydrolysis products corresponding to these compounds by sodium hydroxide (1 N NaOH) treatment. The conventional and the synchronous fluorescence spectra of these hydrolyzed products were totally overlapped making resolving of this mixture impossible. The second-derivative constant-wavelength synchronous fluorescence spectra allowed their detection simultaneously in a single scan after experimental conditions optimization, which was measured at 390 nm and 379 nm for cefadroxil and cefradine, respectively at ?? = 30.0. The calibration curves between derivative synchronous fluorescence intensity and drugs concentration showed suitable linear correlation in the range of 0.1 to 5 ?g.mL? 1 for cefadroxil and 0.5�10 ?g.mL? 1 for cefradine. The proposed fluorimetric method is superior in being simple, environmental friendly and cost effective in comparison to the previously published reported methods. � 2019 Elsevier B.V.EnglishCefadroxilCefradine and water samplesDerivative synchronous spectrofluorimetryCost effectivenessFluorescence spectroscopyHydrolysisSodium hydroxideCefadroxilEnvironmental-friendlyExperimental conditionsHydrolysis productsSimultaneous detectionSpectrofluorimetrySynchronous fluorescenceWater samplesFluorescenceArticlehttps://doi.org/10.1016/j.saa.2019.117903PubMed ID :