Mahmoud B.K.Hamed A.N.E.Samy M.N.Abdelmohsen U.R.Attia E.Z.Fawzy M.A.Refaey R.H.Salem M.A.Pimentel-Elardo S.M.Nodwell J.R.Desoukey S.Y.Kamel M.S.Department of PharmacognosyFaculty of PharmacyMinia UniversityMiniaEgypt; Department of PharmacognosyFaculty of PharmacyDeraya UniversityNew Minia CityEgypt; Departmentof BiochemistryFaculty of PharmacyMinia UniversityMiniaEgypt; Department of Pharmaceutical ChemistryFaculty of PharmacyOctober University of Modern Sciences and Arts (MSA)GizaEgypt; Department of BiochemistryUniversity of TorontoTorontoONCanada2020-01-092020-01-09201914786419https://doi.org/10.1080/14786419.2019.1698571PubMed ID 31797686https://t.ly/7OGVAScopusBoth ethyl acetate and aqueous fractions of Tabebuia aurea leaves exhibited noteworthy antioxidant and nephroprotective activities against carbon tetrachloride (CCl4)-induced nephrotoxicity in rats, as evidenced by the remarkable improvements of renal serum biomarkers and histopathological features. Additionally, the ethyl acetate fraction displayed a prominent in vitro antitrypanosomal activity against Trypanosoma brucei; consequently, the leaves were subjected to LC-HR-ESI-MS metabolomic profiling to discover the constituents that possibly underlie their bioactivities. Therefore, ten metabolites were characterized, mostly dominated by flavonoids. Interestingly, two identified constituents viz., 3,9,12,15-octadecatetraenoic acid (9) and 9,11,13-octadecatrienoic acid (10) are reported firstly herein from the genus Tabebuia. Furthermore, among the dereplicated constituents, rutin (5) and kaempferol 3-O-rutinoside (6) exhibited the highest docking scores as effective antitrypanosomal compounds. � 2019, � 2019 Informa UK Limited, trading as Taylor & Francis Group.EnglishantioxidantantitrypanosomalBignonia aureametabolomic profilingnephroprotectiveTabebuia argenteaTabebuia aureaArticlehttps://doi.org/10.1080/14786419.2019.1698571PubMed ID 31797686