Youness R.A.Assal R.A.Ezzat Shahira MGad M.Z.Abdel Motaal A.Department of Pharmaceutical BiologyFaculty of Pharmacy and BiotechnologyGerman University in CairoCairoEgypt; Department of Pharmacology and ToxiciologyFaculty of Pharmacy and BiotechnologyGerman University in CairoCairoEgypt; Department of PharmacognosyFaculty of PharmacyCairo UniversityGizaEgypt; Department of PharmacognosyFaculty of PharmacyModern Sciences and Arts University6th of OctoberEgypt; Department of BiochemistryFaculty of Pharmacy and BiotechnologyGerman University in CairoCairoEgypt; Department of PharmacognosyCollege of PharmacyKing Khalid UniversityAbhaSaudi Arabia2020-01-092020-01-09201814786419https://doi.org/10.1080/14786419.2018.1509326PubMed ID :https://t.ly/W5Ez9ScopusMSA Google ScholarThis study focused on studying the impact of flavonoids isolated from Cleome droserifolia on HCC cell lines and to further unveil their possible impact on TP53 and its downstream tumor suppressor miRNAs. Three flavonol glycosides were isolated from C. droserifolia namely, Isorhamnetin-3-O-?-D-glucoside (1), Quercetin-3`-methoxy-3-O-(4``-acetylrhamnoside)-7-O-?-rhamnoside (2), and Kaempferol-4`-methoxy-3,7-O-dirhamnoside (3). They showed a concentration and time dependent reduction in cellular viability and anchorage-independent growth of HCC cells. Moreover, they exhibited a decrease in the migrating capacity of HepG2 cells in a pattern similar to positive control cells. (2) Showed the most potent effects in halting HCC tumorigenic activity (IC50=36 � 1.70 �M) and a repression of the cellular proliferation rate of HepG2 cells. Restoration of TP53 and its downstream tumor suppressor miRNAs; miR-15a, miR-16, miR-34a by (2) was observed. Moreover, attenuation of (2) mediated actions was shown upon using anti-miR-15a and anti-miR-16. To conclude, this study crystallizes a novel role of C. droserifolia in harnessing HCC progression in-vitro with a possible contribution of TP53/miR-15a/miR-16. � 2018, � 2018 Informa UK Limited, trading as Taylor & Francis Group.EnglishCleome droserifoliaflavonoidshepatocelllar carcinomamiR-15amiR-16QuercetinTP53Article in Presshttps://doi.org/10.1080/14786419.2018.1509326PubMed ID :