Abdel-Hafiz S.M.Hamdy H.E.Khorshed F.M.Aboushousha T.S.Safwat G.Saber M.A.Seleem M.Soliman A.H.Theodore Bilharz Research Institute (TBRI)Egypt; Faculty of BiotechnologyOctober University of Modern Sciences and ArtsEgypt; Theodore Bilharz Research Institute El-WarrakEmbaba GizaEgypt; National Hepatology and Tropical Medicine Institute (NHTMRI)Egypt; National Cancer InstituteCairo UniversityCairoEgypt2020-01-092020-01-09201815137368https://doi.org/10.22034/APJCP.2018.19.4.1021PubMedID29693976https://t.ly/LXXx0ScopusMSA Google ScholarBackground: Hepatocellular carcinoma (HCC) is a high incidence disease in Egypt with a poor prognosis and survival. Biomarkers are important for diagnosis of HCC at an early stage. Osteopontin (OPN), a glycoprotein secreted by macrophages, osteoblasts, and T cells, is also highly expressed in a variety of tumors, such as examples in the breast, colon, and stomach. The present study aimed to correlate the serum level of OPN in HCV-positive hepatocellular carcinoma patients, with OPN expression in tumor and non-tumor liver tissues in order to identify its efficacy as a biomarker for diagnosis. Material and Methods: Out of total of 146 patients, 80 were selected for inclusion in the study. Blood samples as well as specimens of tumor and non-tumor liver tissue were collected. In addition, blood samples from 20 healthy volunteers were obtained as controls. Serum OPN and alpha-fetoprotein (AFP) were evaluated by ELISA for HCC and control groups. OPN and AFP gene expression were examined by real-time PCR, after homogenization and DNA extraction from serum samples and liver tissues. Results: It was found that serum OPN levels were significantly higher in the HCC group compared to normal group (P=0.009), with a strong positive correlation with AFP expression. However, there was no significant difference between OPN expression in tumor and non-tumor liver tissue. Conclusion: Serum OPN is highly suggested to be a professional candidate for HCC early diagnosis, with a diagnostic ability and accuracy equal or higher than for AFP. � 2017, Asian Pacific Organization for Cancer Prevention.EnglishOctober University for Modern Sciences and ArtsUniversity for Modern Sciences and ArtsMSA Universityجامعة أكتوبر للعلوم الحديثة والآدابAlpha-fetoproteinHepatocellular carcinomaOsteopontinReal-time PCRalpha fetoproteinosteopontinSPP1 protein, humantumor markeradultbloodcase control studychronic hepatitis Ccomplicationevaluation studyfemalefollow upHepacivirushumanisolation and purificationliverliver cell carcinomaliver tumormalemetabolismmiddle agedpathologyprognosisreceiver operating characteristicvirologyAdultalpha-FetoproteinsBiomarkers, TumorCarcinoma, HepatocellularCase-Control StudiesFemaleFollow-Up StudiesHepacivirusHepatitis C, ChronicHumansLiverLiver NeoplasmsMaleMiddle AgedOsteopontinPrognosisROC CurveArticlehttps://doi.org/10.22034/APJCP.2018.19.4.1021PubMedID29693976