Mohamed A.-M.I.Abdelmageed O.H.Salem H.Nagy D.M.Omar M.A.Pharmaceutical Analytical Chemistry DepartmentFaculty of PharmacyAssiut UniversityAssiutEgypt; Pharmaceutical Chemistry DepartmentFaculty of PharmacyKing Abdulaziz UniversityJeddahSaudi Arabia; Pharmaceutical Analytical Chemistry DepartmentFaculty of PharmacyMSA UniversityCairoEgypt; Analytical Chemistry DepartmentFaculty of PharmacyMinia UniversityMiniaEgypt2020-01-092020-01-09201315227235https://doi.org/10.1002/bio.2388PubMed ID 22786713https://t.ly/BX3jMScopusA validated simple and sensitive spectrofluorimetric method was developed for the determination of chlorpromazine hydrochloride, promethazine hydrochloride, trifluperazine hydrochloride, thioridazine hydrochloride, perazine maleate and oxomemazine. The method was based on condensation of malonic acid/acetic anhydride (MAA) under the catalytic effect of the tertiary amine moiety of the studied phenothiazines to provide a deep yellow to brown colour with green florescence. Relative fluorescence intensity of the products was measured at ?exc 398 nm and ?em 432 nm. Different variables affecting the reaction were studied and optimized. The method was successfully applied for the determination of the studied drugs in commercial dosage forms. The lower detection limits allowed the application of this method for the determination of the compounds in plasma as an example of a biological fluid. In addition, the method was considered specific for the determination of tertiary amines in the presence of primary and secondary amines; as a result, it was deemed suitable for the determination of the cited drugs in the presence of their degradation products resulting from N-dealkylation or oxidation of the corresponding sulphoxides or sulphones. Copyright � 2012 John Wiley & Sons, Ltd.EnglishOctober University for Modern Sciences and ArtsUniversity for Modern Sciences and ArtsMSA Universityجامعة أكتوبر للعلوم الحديثة والآدابacetic anhydridedosage formsmalonic acidphenothiazinesplasmaspectrofluorimetric determinationdrug dosage formneuroleptic agentphenothiazine derivativeneuroleptic agentphenothiazine derivativeblooddrug dosage formevaluation studyhumanproceduresspectrofluorometryarticlemethodologyspectrofluorometryAntipsychotic AgentsDosage FormsHumansPhenothiazinesSpectrometry, FluorescenceAntipsychotic AgentsDosage FormsHumansPhenothiazinesSpectrometry, FluorescenceArticlehttps://doi.org/10.1002/bio.2388PubMed ID 22786713