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Cellini L, Di Campli E, Masulli M, Di Bartolomeo S, Allocati N. Inhibition of Helicobacter pylori by garlic extract (Allium sativum). FEMS Immunol Med Microbiol. 1996; 13: 273-7. 33. Groppo FC, Ramacciato JC, Simoes RP, Florio FM, Sartoratto A. Antimicrobial activity of garlic, tea tree oil, and chlorhexidine against oral microorganisms. Intern Dent J 2002; 52: 433-437. 34. Perez-Giraldo C, Cruz-Villalon G, Sanchez-Silos R, Martinez- Rubio R, Blanco MT, Gomez-Garcia AC. In vitro activity of allicin on Staphylococcus epidermidis and influence of subinhibitory concentrations on biofilm formation. J App Microbiol 2003; 95: 709-11.2278 – 4357https://doi.org/http://central-library.msa.edu.eg:8009/xmlui/handle/123456789/420Irrigation of the root canals with antibacterial solutions is a mandatory step to reduce or eliminate micro-organisms. The aim of this study was to evaluate the efficacy of garlic and ginger as irrigating solutions. The ethanolic extracts of garlic bulbs, ginger rhizomes and 2% solution of CHX were prepared. Forty single rooted teeth canal were divided into 3 groups according to the irrigant used, in addition to the positive control group where the roots were irrigated with sterile distilled water and a negative control group in which the roots were uninfected with E. faecalis. The teeth were filled with 30 μl of E. faecalis suspension and incubated for 1 week at 37ºC. They were prepared mechanically using protaper rotary files both shaper and finisher, each root was irrigated successively with 1 ml of the tested irrigant after each file and then 3 ml as a final flush. After chemo- mechanical preparation, samples were evaluated for antibacterial evaluation using brain heart infusion agar method. The components of the ethanolic extracts of garlic bulbs and ginger rhizomes were identified using LC/ESI-MS analysis. The highest antibacterial activity was observed in ginger extract followed by 2% CHX solution, and garlic extract. Zingerone, gingerol and [6]-shogaol were the major components determined in ginger extract using LC/ESI-MS, while garlic extract was rich in di-2-propenyldisulfide, methyl-2-propenyl trisulfide, 3-vinyl-1,2-dithiocyclohex-4-ene and di-2-propenyltrisulfide. The ethanolic extracts of both garlic bulbs and ginger rhizomes can be used as endodontic irrigants during the root canal treatment for their antimicrobial effects.enUniversity of Enterococcus faecalisArticlehttps://doi.org/