Moustafa A.A.Hegazy M.A.Mohamed D.Ali O.Analytical Chemistry DepartmentFaculty of PharmacyCairo UniversityKasr-El Aini StreetCairo11562Egypt; Analytical Chemistry DepartmentFaculty of PharmacyOctober University for Modern Sciences and Arts (MSA)6th October City11787Egypt; Analytical Chemistry DepartmentFaculty of PharmacyHelwan UniversityEin HelwanCairo11795Egypt2020-01-092020-01-09201613861425https://doi.org/10.1016/j.saa.2015.10.010PubMed ID 26519913https://t.ly/3G0JwScopusA novel approach for the resolution and quantitation of severely overlapped quaternary mixture of carbinoxamine maleate (CAR), pholcodine (PHL), ephedrine hydrochloride (EPH) and sunset yellow (SUN) in syrup was demonstrated utilizing different spectrophotometric assisted multivariate calibration methods. The applied methods have used different processing and pre-processing algorithms. The proposed methods were partial least squares (PLS), concentration residuals augmented classical least squares (CRACLS), and a novel method; continuous wavelet transforms coupled with partial least squares (CWT-PLS). These methods were applied to a training set in the concentration ranges of 40-100 ?g/mL, 40-160 ?g/mL, 100-500 ?g/mL and 8-24 ?g/mL for the four components, respectively. The utilized methods have not required any preliminary separation step or chemical pretreatment. The validity of the methods was evaluated by an external validation set. The selectivity of the developed methods was demonstrated by analyzing the drugs in their combined pharmaceutical formulation without any interference from additives. The obtained results were statistically compared with the official and reported methods where no significant difference was observed regarding both accuracy and precision. 2015 Elsevier B.V. All rights reserved.EnglishCarbinoxamineContinuous wavelet transforms coupled with partial least squares (CWT-PLS)Cyrinol� syrupEphedrineMultivariate calibration methodsPholcodineAlgorithmsCalibrationDrug productsMixturesMultivariable systemsWavelet transformsCarbinoxamineEphedrineMultivariate calibration methodsPartial least square (PLS)PholcodineLeast squares approximationsazo compoundcarbinoxaminecodeineephedrineFD & C Yellow No. 6morpholine derivativepholcodinepyridine derivativealgorithmanalogs and derivativescalibrationevaluation studyleast square analysismultivariate analysisproceduresspectrophotometryAlgorithmsAzo CompoundsCalibrationCodeineEphedrineLeast-Squares AnalysisMorpholinesMultivariate AnalysisPyridinesSpectrophotometryArticlehttps://doi.org/10.1016/j.saa.2015.10.010PubMed ID 26519913