Farid, MuhammadArif, MakrufPrihantoko, Kurniawan DwiKusumawati, AsmaraniWijayanti, Agustina DwiMaha, ErifSetyawan, Nugraha2021-07-302021-07-306/15/2021https://doi.org/10.17582/JOURNAL.AAVS/2021/9.7.1034.1039https://qrgo.page.link/wVXxgThe study aims to determine the effects of vitamin C, vitamin E, and the combinations of vitamin C and E supplements on sperm quality of KUB Chicken for 4, 8, 24, and 48 hours in 4 °C at cold storage. A total of twelve KUB chickens were used in this study. The semen was collected by the dorso-abdominal massage method twice a week and diluted with Ringer’s modified solution. The treatments were divided into four different treatment actions (P), the control group (P0) as neutral without supplement added, and the treatments group. The treatments were divided into three different groups, added vitamin C 200 µg/ml (P1), the vitamin E 5 µg/ml (P2), and combinations of vitamin C 200 µg/ml + vitamin E 5 µg/ml (P3). Evaluation rate included sperm motility, recovery rate, viability, membrane integrity, and DNA fragmentation after 2 (T1), 4 (T2), 8 (T3), 24 (T4), and 48 (T5) hours stored at 4°C. Whereas, the evaluation of motility, viability, and membrane integrity of T1, T2, and T3 storage showed no significant difference between treatment groups (P> 0.05), T4 and T5 in treatment group P2 were significantly higher (P<0.05) and P3 were lower (P<0.05) than the other treatment groups. DNA fragmentation examination on fresh semen at T5 storage showed no significant difference between treatment groups (P >0.05). This study concluded that vitamin E 5 µg/ml supplement bests to improve and extend the motility, viability, membrane integrity, and the combination of vitamin C and vitamin E may decrease the sperm quality KUB chickens up to 48 hours storage at 4°C. Copyright© 2021 Kusumawati et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en-USDNA FragmentationKUB chickenSemen QualityVitamin CVitamin EArticlehttps://doi.org/10.17582/JOURNAL.AAVS/2021/9.7.1034.1039