Abd El-Fatah, Nourhan AEl-Sayed, Ghada MHegazy, Maha AFouad, Manal MohammedElbalkiny, Heba T2023-10-072023-10-072023-0810.1149/1945-7111/acef5a]http://repository.msa.edu.eg/xmlui/handle/123456789/5738Tigecycline (TGC) is a novel potent antibiotic with recently proven anticancer activity against leukemia, glioma, and lung cancer. In-line TGC potentiometric sensors are fabricated for monitoring TGC in its pure form, pharmaceutical formulation, presence of its degradation products, and spiked human plasma. In-line sensors act as greener, portable, and economical alternatives to the classical off-line separation-based techniques. Classical and advanced liquid-contact (LC) and solid-contact (SC) sensors were fabricated, where the best performance was observed with the modified SC sensor (sensor VI) with potassium tetrakis (4- chlorophenyl) borate as ionic exchanger, β-cyclodextrin ionophore and cobalt oxide nanoparticles, showing a Nernstian response of 30 mV decade−1 in the linear range of 10−2 –10−6 M. Statistical comparison was carried out for the results obtained from proposed SC sensors and the official method on TGC pure form. Additionally, method greenness was evaluated using a semi-quantitative analytical eco-scale, scoring approximately 95 points, which was the highest greenness achievement score when compared to the proposed LC sensors or British Pharmacopeial chromatographic method.endegradation products; green profile; liquid-contact; nanoparticle; potentiometric; solid-contact; spiked plasma; tigecyclineArticle10.1149/1945-7111/ acef5a