Emara S.Masujima T.Zarad W.Mohamed K.Kamal M.Fouad M.EL-Bagary R.Faculty of PharmacyPharmaceutical Chemistry DepartmentMisr International UniversityKm 28 Ismailia RoadCairoEgypt; P.I. Laboratory Single Cell MSRIKEN Quantitative Biology Center6-2-3FuruedaiSuitaOsaka565-0874Japan; Faculty of PharmacyPharmacognosy DepartmentAssiut UniversityAssiut71526Egypt; Faculty of PharmacyPharmaceutical Analytical Chemistry DepartmentModern Sciences and Arts University26 July Mehwar Road Intersection with Wahat Road6 October CityEgypt; Faculty of PharmacyPharmaceutical Chemistry DepartmentCairo UniversityKasr El Aini St.Cairo11562Egypt2020-01-092020-01-092014219665https://doi.org/10.1093/chromsci/bmt169PubMed ID 24248558https://t.ly/lWOEDScopusSuccessful simultaneous diastereomeric separation and sensitive determination of two pairs of triterpenoidal saponins have been achieved by capillary electrophoresis (CE) using ?-cyclodextrin (?-CD) as a stereoselective agent to cooperate with borate complexation. A usual technique for isolation and group separation of saponins was developed as an appropriate purification step prior to the determination of individual saponins by CE. Soyasaponin I (S1), azukisaponin V (S2), bersimoside I (S3) and bersimoside II (S4) could be well separated within 14 min in a fused-silica capillary (60 cm long to the detector with an additional 10 cm to the cathode; 75 ?m i.d.). The background electrolyte was borate buffer (80 mM, pH 10), containing 24 mM ?-CD. The separation voltage was 14 kV with a detection wavelength of 195 nm. The sample was electrokinetically injected using a voltage of 16 kV for 12 s. Methanol (70%) was used as the diluent for field-amplified sample stacking after hydrodynamic injection of short water plug (5 cm, 4 s). The method was partially validated for linearity, repeatability, reproducibility, limits of detection and limits of quantification. The correlation coefficients of the calibration curves were all >0.998, and the recoveries were from 98.23 to 96.21%. The Author [2013]. Published by Oxford University Press. All rights reserved.EnglishOctober University for Modern Sciences and Artsجامعة أكتوبر للعلوم الحديثة والآدابUniversity of Modern Sciences and ArtsMSA Universitybeta cyclodextrin derivativeboric acidplant extractsaponincapillary electrophoresischemistrycloverlimit of detectionpHplant seedproceduresreproducibilitystatistical modelbeta-CyclodextrinsBoratesElectrophoresis, CapillaryHydrogen-Ion ConcentrationLimit of DetectionLinear ModelsPlant ExtractsReproducibility of ResultsSaponinsSeedsTrifoliumArticlehttps://doi.org/10.1093/chromsci/bmt169PubMed ID 24248558