Saber, AyaAbdelaziz Ibrahim, SherifHosney, MohamedTaha Mohamed, HossamFares, MohamedSabet, SalwaEl-Shinawi, MohamedMostafa Mohamed, Mona2020-02-082020-02-082019Almuhanna, M. A., Alsaif, M., Alsaadi, M., & Almajwal, A. (2014). Fast food intake and prevalence of obesity in school children in Riyadh City. Sudanese Journal of Paediatrics, 14(1), 71– 80. Bailey, K. M., & Liu, J. (2008). Caveolin-1 up-regulation during epithelial to mesenchymal transition is mediated by focal adhesion kinase. Journal of Biological Chemistry, 283(20), 13714–13724. https://doi.org/ 10.1074/jbc.M709329200 Bouchal, P., Schubert, O. T., Faktor, J., Capkova, L., Imrichova, H., Zoufalova, K., … Aebersold, R. (2019). Breast Cancer Classification Based on Proteotypes Obtained by SWATH Mass Spectrometry. Cell Reports, 28(3), 832–843.e7. https:// doi. org/10.1016/j.celrep.2019.06.046 Catalán, V., Gómez-ambrosi, J., Rodríguez, A., Silva, C., Rotellar, F., Gil, M. J., … Frühbeck, G. (2008). Expression of caveolin-1 in human adipose tissue is upregulated in obesity and obesity-associated type 2 diabetes mellitus and related to inflammation, 948255400, 213– 219.https://doi.org/10.1111 /j.1365 -2265.2007.03021.x Chan, D. S. M., Sc, M., Norat, T., & Ph, D. (2015). Obesity and Breast Cancer : Not Only a Risk Factor of the Disease. https://doi.org/ 10.1007/s11864-015-0341-9 Chang, C., Chen, C., Wen, H., Huang, C., Hung, M., & Lu, H. (2017). Caveolin-1 Secreted from Adipose Tissues and Adipocytes Functions as an Adipogenesis Enhancer, 25(11), 1932–1940. https://doi.org/10.1002/oby.21970 Chu, D., Nguyen, T., Phuong, T., Le, N., Tien, B., & Tran, D. (2019). cells The E ff ects of Adipocytes on the Regulation of Breast Cancer in the Tumor Microenvironment : An Update, 1–19. Cohen, A. W., Hnasko, R., Schubert, W., & Lisanti, M. P. (2004). Role of caveolae and caveolins in health and disease. Physiological Reviews, 84(4), 1341–1379. https://doi.org/10.1152/physrev.0 0046.2003 Engin, A. B., Engin, A., & Gonul, I. I. (2019). The effect of adipocyte – macrophage crosstalk in obesityrelated breast cancer. Gai, X., Lu, Z., Tu, K., Liang, Z., & Zheng, X. (2014). Caveolin-1 is up-regulated by GLI1 and contributes to GLI1-driven EMT in hepatocellular carcinoma. PLoS ONE, 9(1). https://doi.org/ 10.1371/journal.pone.0084551 Goetz, J. G., Lajoie, P., Wiseman, S. M., & Nabi, I. R. (2008). Caveolin-1 in tumor progression: The good, the bad and the ugly. Cancer and Metastasis Reviews, 27(4), 715–735. https://doi.org/ 10.1007/s10555-008-9160-9 Guerro-Millo, M. (2004). Adipose tissue and adipokines: For better or worse. Diabetes and Metabolism, 30(1), 13–19. https: //doi.org/10.1016/s1262-3636 (07) 70084-8 Ibrahim, A. S., Khaled, H. M., Mikhail, N. N. H., Baraka, H., & Kamel, H. (2014). Cancer Incidence in Egypt : Results of the National Population-Based Cancer Registry Program, 2014. MOKBEL, K., & MOKBEL, K. (2019). Chemoprevention of Breast Cancer With Vitamins and Micronutrients: A Concise Review. In Vivo, 33(4), 983–997. https://doi.org/10.21873/invivo.11 568 Nielsen, T. O., Hsu, F. D., Jensen, K., Cheang, M., Karaca, G., Hu, Z., … Perou, C. M. (2004). Immunohistochemical and Clinical Characterization of the Basal- Like Subtype of Invasive Breast Carcinoma, 10(919), 5367–5374. Nouh, M. A., Mohamed, M. M., ElShinawi, M., Shaalan, M. A., Cavallo-Medved, D., Khaled, H. M., & Sloane, B. F. (2011). Cathepsin b: A potential prognostic marker for inflammatory breast cancer. Journal of Translational Medicine, 9(1), 1. https://doi.org/ 10.1186/1479-5876-9-1 Nwosu, Z. C., Ebert, M. P., Dooley, S., & Meyer, C. (2016). Caveolin-1 in the regulation of cell metabolism : a cancer perspective. Molecular Cancer, 1–12. https:// doi.org/10.1186/s12943-016- 0558-7 Podar, K., Tai, Y., Cole, C. E., Hideshima, T., Sattler, M., Hamblin, A., … Anderson, K. C. (2003). Essential Role of Caveolae in Interleukin-6- and Insulin-like Growth Factor Itriggered Akt-1-mediated Survival of Multiple Myeloma Cells *, 278(8), 5794–5801. https://doi.org/10.1074/jbc.M208 636200 Qian, X. L., Pan, Y. H., Huang, Q. Y., Shi, Y. B., Huang, Q. Y., Hu, Z. Z., & Xiong, L. X. (2019). Caveolin-1: A multifaceted driver of breast cancer progression and its application in clinical treatment. OncoTargets and Therapy, 12, 1539–1552. https:// doi.org/10.2147/OTT.S191317 Shuch, Brian; Linehan, B. W. M.L.; Srivasan, R. (2012). O ncologist G, 1051–1062. https://doi.org/ 10.1634/theoncologist.2011-0227 Tahir, S. A., Yang, G., Ebara, S., Timme, T. L., Satoh, T., Li, L., … Thompson, T. C. (2001). Secreted caveolin-1 stimulates cell survival/clonal growth and contributes to metastasis in androgen-insensitive prostate cancer. Cancer Research, 61(10), 3882–3885. Tiwari, P., Blank, A., Cui, C., Schoenfelt, K. Q., Zhou, G., Xu, Y.,Olopade, F. (2019). Metabolically activated adipose tissue macrophages link obesity to triple-negative breast cancer, 216(6), 1345–1358. Torre, L. A., Bray, F., Siegel, R. L., & Ferlay, J. (2015). Global Cancer Statistics , 2012, 65(2), 87–108. https://doi.org/10.3322/caac.2126 2. Victor, B. C., Anbalagan, A., Mohamed, M. M., Sloane, B. F., & Cavallo-medved, D. (2011). Inhibition of cathepsin B activity attenuates extracellular matrix degradation and inflammatory breast cancer invasion. Breast Cancer Research, 13(6), R115. https://doi.org/10.1186/bcr3058 Williams, T. M., & Lisanti, M. P. (2004). The caveolin proteins. Genome Biology, 5(3), 1–8. https://doi.org/10.1186/gb-2004- 5-3-214 Williams, T. M., Sotgia, F., Lee, H., Hassan, G., Di Vizio, D., Bonuccelli, G., … Lisanti, M. P. (2006). Stromal and epithelial caveolin-1 both confer a protective effect against mammary hyperplasia and tumorigenesis: Caveolin-1 antagonizes cyclin D1 function in mammary epithelial cells. American Journal of Pathology, 169(5), 1784–1801. https: //doi.org/10.2353/ajpath.2006.060 590 Yedjou, C. G., Sims, J. N., Miele, L., Noubissi, F., Lowe, L., Fonseca, D. D., … Tchounwou, P. B. (2019). Health and Racial Disparity in Breast Cancer. Advances in Experimental Medicine and Biology, 1152, 31– 49. https://doi.org/10.1007/978-3- 030-20301-6_3 Yehia Ibrahim, N. (2019). ClinicoEpidemiological Study of Elderly Breast Cancer in a Developing Country: Egypt. Journal of Cancer Treatment and Research, 7(1), 23. https://doi.org/ 10.11648/ j.jctr.20190701.14.2090 – 0775https://doi.org/10.21608/EAJBSD.2020.69041https://t.ly/r7rm0MSA Google ScholarBreast cancer (BC) is the second leading mortality cause due to poor survival rates compared to lung cancer all over the world. Recently, lifestyle increased obesity among the population globally. Since, the adipose tissues (AT) are the major contributor to the volume of the breast and adipocytes cells, which constitute AT are one of the major prominent cells play an effective role in cancer progression via releasing different mediators and adipokines. Thus, AT may display a crucial role in BC progression, especially in obese patients compared to non-obese patients, which characterized by increased AT. Interestingly, adipocytes are characterized by expressing caveolin-1 (Cav-1) protein. Cav-1 constitutes the lipid raft of caveola which contains different proteolytic enzymes inducing cancer metastasis. In this regard, the aim of the present study was to explore the level of expression of Cav-1 protein in the tissue specimen of 5 non-obese vs. 15 obese patients using immunohistochemistry (IHC) and immunoblotting techniques. Our finding demonstrates that the level of Cav-1expression was statistically significantly low in non-obese compared to obese BC patients (p < 0.05). Herein, our results revealed that the highest expression of Cav-1 in obese patients compared to non-obese (control) patients can be considered as a biomarker for BC patients.enBreast canceradipose tissuesArticlehttps://doi.org/10.21608/EAJBSD.2020.69041