Emara S.Masujima T.Zarad W.Kamal M.Fouad M.El-Bagary R.Pharmaceutical Chemistry DepartmentFaculty of PharmacyMisr International UniversityKm 28 Ismailia RoadCairoEgypt; P.I. Lab. Single Cell MSRIKEN Quantitative Biology Center6-2-3FuruedaiSuitaOsaka 565-0874Japan; Pharmaceutical Analytical Chemistry DepartmentFaculty of PharmacyModern Sciences and Arts University26 July Mehwar Road Intersection With Wahat Road6 October CityEgypt; Pharmaceutical Chemistry DepartmentFaculty of PharmacyCairo UniversityKasr El Aini St.Cairo11562Egypt2020-01-092020-01-092015219665https://doi.org/10.1093/chromsci/bmv024PubMed ID 25834172https://t.ly/NXMvxScopusA simple, rapid and environment-friendly direct injection HPLC method for the determination of methyldopa (MTD) in human serum has been developed and validated. The method was based on cleanup and separation of MTD from serum by mixed-mode liquid chromatography using a single protein-coated TSK gel ODS-80 TM analytical column (50 x 4.0 mm i.d., 5 ?m). The protein-coated column exhibited excellent resolution, selectivity and functioned in two chromatographic modes: size-exclusion chromatography [i.e., solid-phase extraction (SPE) for serum proteins] and reversed-phase chromatography for the final separation of MTD. SPE and HPLC separation were carried out simultaneously with a green mobile phase consisting of acetate buffer (0.1 M, pH 2.4) at a flow rate of 1 mL/min and at room temperature (23 � 1�C). The eluent was monitored at emission and excitation wavelengths of 320 and 270 nm, respectively. A calibration curve was linear over the range of 0.1-30 ?g/mL with a detection limit of 0.027 ?g/mL. This online SPE method was successfully applied to real samples obtained from patients receiving MTD therapy. � The Author 2015. Published by Oxford University Press. All rights reserved.EnglishOctober University for Modern Sciences and ArtsUniversity for Modern Sciences and ArtsMSA Universityجامعة أكتوبر للعلوم الحديثة والآدابmethyldopaadultblooddeviceshigh performance liquid chromatographyhumanlimit of detectionmaleproceduresreproducibilitysize exclusion chromatographysolid phase extractionstatistical modelAdultChromatography, GelChromatography, High Pressure LiquidHumansLimit of DetectionLinear ModelsMaleMethyldopaReproducibility of ResultsSolid Phase ExtractionArticlehttps://doi.org/10.1093/chromsci/bmv024PubMed ID 25834172