El-Ekiaby N.M.Mekky R.Y.Riad S.E.Elhelw D.S.El-Sayed M.Esmat G.Abdelaziz A.I.Department of Pharmacology and ToxicologyMolecular Pathology Research GroupGerman University in CairoNew Cairo City Main Entrance Al Tagamoa Al KhamesCairoEgypt; Scientific Affairs UnitEgyptian Company for Biological SciencesGizaEgypt; Faculty of BiotechnologyOctober University of Modern Sciences and ArtsGizaEgypt; Endemic Medicine and HepatologyCairo UniversityCairoEgypt; Biotechnology Graduate ProgramAmerican University in CairoAUC AvenueNew CairoCairoEgypt2020-01-092020-01-0920171466615https://doi.org/10.1002/jmv.24677PubMedID27591428https://t.ly/DXGn2ScopusHepatitis C Virus (HCV) promotes lipid droplet (LD) formation and perturbs the expression of the LD associated PAT proteins ADRP and TIP47, to promote its own lifecycle. HCV enhances TIP47 and suppresses ADRP by displacing it from LD surface in infected cell models. We have previously shown that suppression of TIP47 by miR-148a and miR-30a decreased intracellular LDs and HCV RNA. Thus, this study aimed at examining whether this microRNA-mediated suppression of HCV would limit HCV-dependent displacement of ADRP from LDs. ADRP expression was examined in 21 HCV-infected liver biopsies and 9 healthy donor liver tissues as well as in HCV-infected Huh7 cells using qRT-PCR. miR-148a and miR-30a expression was manipulated using specific oligos in JFH-1 infected, oleic acid treated cells, to study their impact on ADRP expression using qRT-PCR, and immunofluorescence microscopy. Intracellular HCV RNA was assessed using qRT-PCR. ADRP is down regulated in patients as well as HCVcc-JFH-I infected cell models. Forcing the expression of both miRNAs induced ADRP on the mRNA and protein levels. This study shows that HCV suppresses hepatic ADRP expression in infected patients and cell lines. Forcing the expression of miR-148a and miR-30a limits the suppressive effect of HCV on ADRP. J. Med. Virol. 89:653 659, 2017. 2016 Wiley Periodicals, Inc. 2016 Wiley Periodicals, Inc.EnglishlipidslivermicroRNAsPAT proteinsviral infectionadipophilinmannose 6 phosphate receptor binding protein 1microRNAmicroRNA 148amicroRNA 30aunclassified drugvirus RNAadipophilinmannose 6 phosphate receptor binding protein 1microRNAMIRN148 microRNA, humanMIRN30 microRNA, humanPLIN2 protein, humanPLIN3 protein, humanvirus RNAADRP geneadultagedArticleclinical articlecontrolled studydown regulationfemalegene expressiongene functionhepatitis Chumanhuman cellhuman tissueimmunofluorescence microscopymalemiddle agedprotein expressionreverse transcription polymerase chain reactionantagonists and inhibitorsbiopsycell linefluorescence microscopygene expression profilingHepacivirushepatitis Chost pathogen interactionliverliver cellmetabolismpathogenicitypathologyreal time polymerase chain reactionvirologyAdultBiopsyCell LineFemaleGene Expression ProfilingHepacivirusHepatitis CHepatocytesHost-Pathogen InteractionsHumansLiverMaleMicroRNAsMicroscopy, FluorescenceMiddle AgedPerilipin-2Perilipin-3Real-Time Polymerase Chain ReactionRNA, ViralArticlehttps://doi.org/10.1002/jmv.24677PubMedID27591428