El-Khazragy N.Elayat W.Matbouly S.Seliman S.Sami A.Safwat G.Diab A.Clinical Pathology and Hematology DepartmentFaculty of MedicineAin Shams University Biomedical Research DepartmentP.O. Box 11381CairoEgypt; Department of Medical BiochemistryFaculty of MedicineAin Shams UniversityEgypt; Department of PediatricsFaculty of MedicineAin Shams UniversityEgypt; Faculty of BiotechnologyOctober University for Modern Sciences and Arts (MSA)CairoEgypt2020-01-092020-01-0920193781119https://doi.org/10.1016/j.gene.2019.03.055PubMedID30943439https://t.ly/veqn7ScopusMSA Google ScholarLong non-coding RNA (LncRNA) is recently linked to various types of cancers, CCAT and PVT1 are two LncRNAs linked to t(8;21) associated Acute Myeloid Leukemia, the interplay between CCAT, PVT1 and the MYC proto-oncogene implicated in t(8;21) could present an opportunity for using LncRNA as prognostic biomarker or a target for therapy, We investigated the expression levels of LncRNAs in 70 patients; 30 with t(8;21) positive AML and 40 with t(8;21) negative AML, We found that CCAT1 and PVT1 are expressed in higher levels in t(8;21) positive �AML by 5.3 folds compared to t(8;21) negative group; the expression values were significantly associated with high-risk clinical criteria; moreover, they are associated with lower overall survival (OS) rate and leukemia-free survival (LFS), however we didn't find a statistically significant cut-off value of LncRNAs using the Cox regression analysis for Lnc_PVT1 except with LFS, we conclude that high expression levels of CCAT1 and PVT1 are associated with poor prognosis while being poor prognostic biomarkers in t(8;21) associated AML. � 2019 Elsevier B.V.EnglishOctober University for Modern Sciences and Artsجامعة أكتوبر للعلوم الحديثة والآدابUniversity of Modern Sciences and ArtsMSA UniversityAcute Myeloid Leukemia (AML)Lnc-CCAT1Lnc-PVT1Prognostic factort(8;21)long non coding RNA CCAT1long non coding RNA PVT1long untranslated RNAtumor markerunclassified drugCCAT1 long noncoding RNA, humanlong untranslated RNAMyc proteinMYC protein, humanPVT1 long-non-coding RNA, humantumor markeracute myeloid leukemiaadultagedArticlecancer prognosiscancer specific survivalclinical featurecohort analysiscomparative studycontrolled studydemographyfemalegene expressiongenetic associationhigh risk patienthumanmajor clinical studymaleoverall survivalpriority journalrisk factorsurvival rateupregulationacute myeloid leukemiachromosome 21chromosome 8gene expression regulationgene translocationgeneticsmiddle agedprognosisregression analysissurvival analysisyoung adultAdultAgedBiomarkers, TumorChromosomes, Human, Pair 21Chromosomes, Human, Pair 8FemaleGene Expression Regulation, NeoplasticHumansLeukemia, Myeloid, AcuteMaleMiddle AgedPrognosisProto-Oncogene Proteins c-mycRegression AnalysisRNA, Long NoncodingSurvival AnalysisTranslocation, GeneticUp-RegulationYoung AdultArticlehttps://doi.org/10.1016/j.gene.2019.03.055PubMedID30943439