Ezzat, Shahira MShouman S.A.Elkhoely A.Attia Y.M.Elsesy M.S.El Senousy A.S.Choucry M.A.El Gayed S.H.El Sayed A.A.Sattar E.A.El Tanbouly N.Pharmacognosy DepartmentFaculty of PharmacyCairo UniversityKasr El-Einy StreetCairo11562Egypt; Department of PharmacognosyFaculty of PharmacyOctober University for Modern Science and Arts (MSA)6th October12566Egypt; Cancer Biology DepartmentNational Cancer InstituteCairo UniversityCairoEgypt; Pharmacology and Toxicology DepartmentFaculty of PharmacyHelwan UniversityHelwanEgypt2020-01-092020-01-09201820452322https://doi.org/10.1038/s41598-017-18944-0PubMed ID 29323210https://t.ly/P5M8XScopusMSA Google ScholarThe objective of our study is to highlight the therapeutic effect and mechanism of action by which purified Flaxseed hydrolysate (PFH) which is a lignan rich fraction exerts its anticancer activity on a human breast cancer cell line (T47D) and in mice bearing tumor. HPLC analysis of PFH of six flaxseed cultivars had shown that PFH of the cultivar Giza 9 (PFH-G9) contains the highest concentration of SDG (81.64 mg/g). The in vitro cytotoxic potentiality of PFH's of six flaxseed cultivars was screened against a panel of human cancer cell lines. PFH -G9 showed the most significant cytotoxic activity against ER-receptor positive breast cell lines MCF7 and T47D with IC50 13.8 and 15.8 ?g/ml, respectively. Moreover, PFH-G9 reduced the expression of the metastasis marker, 1-?, metalloproteinases and vascular endothelial growth factor (VEGF), one of the most potent stimulators of angiogenesis, while it increased the caspase-3 dependent apoptosis. Our study also showed that dietary intake of 10% of Giza 9 Flaxseeds (FS), fixed oil (FSO) or Flax meal (FSM) twice daily for 3 weeks in mice-bearing solid Ehrlich ascites carcinoma (EAC) resulted in reducing the tumor volume, the expression of estrogen, insulin growth factor, progesterone, VEGF and MMP-2, but enhanced expression of caspase-3. � 2018 The Author(s).Englishantineoplastic agentlignanplant extracttumor markeranimalapoptosischemistrydrug effectexperimental mammary neoplasmfemaleflaxgeneticsHCT 116 cell lineHeLa cell linehumanMCF-7 cell linemetabolismmouseAnimalsAntineoplastic AgentsApoptosisBiomarkers, TumorFemaleFlaxHCT116 CellsHeLa CellsHumansLignansMammary Neoplasms, ExperimentalMCF-7 CellsMicePlant ExtractsArticlehttps://doi.org/10.1038/s41598-017-18944-0PubMed ID 29323210