El Tahry F.A.Hashad I.M.Rahman M.F.A.Gad M.Z.Clinical Biochemistry UnitFaculty of Pharmacy and BiotechnologyGerman University in CairoCairoEgypt; Biochemistry DepartmentFaculty of PharmacyOctober University for Modern Science and Arts6th of October CityEgypt2020-01-092020-01-09201713892010https://doi.org/10.2174/1389201018666171002125432PubMed ID 28969560https://t.ly/mb8jAScopusBackground: Connexin (Cx) proteins are the building blocks of gap junctions. Among these, Cx37 and Cx40 are expressed on vascular system and reported to have cardioprotective role. Linking polymorphisms in genes coding for Cx and coronary artery disease (CAD) risk showed conflicting results in different populations. None has been studied before in Egyptians. Therefore, the aims of this study were to investigate the influence of Cx37 C1019T and Cx40 A71G polymorphisms on the predisposition of acute myocardial infarction (AMI) in Egyptians, to study linkage disequilibrium (LD) and combined effects of single nucleotide polymorphisms (SNPs) and to correlate the genotypes with sVCAM-1 serum levels. Methods: Total of 201 Egyptian subjects were recruited for the study. They were divided into 104 AMI patients and 97 healthy controls. Genotypes for each participant were determined using a polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Serum sVCAM-1was measured by ELISA. Results: Allele frequencies for both Cx37 and Cx40 were not significantly different between AMI and Controls (p=0.93 and p=0.26 respectively). Moreover, studying the dominant and recessive models concluded that none of the genotypes was a risk factor. Both SNPs were not in LD (R2=0.0027). Serum analysis showed higher levels of sVCAM-1 in AMI patients (p<0.0001). sVCAM-1 levels were not significantly different among SNPs (Cx37; p=0.244 and Cx40; p=0.266). � 2017 Bentham Science Publishers.EnglishA71G polymorphismC1019T polymorphismConnexin 37Connexin 40EgyptiansGap junctionsMyocardial infarctionSVCAM-1cholesterolgap junction proteintriacylglycerolvascular cell adhesion molecule 1connexin 37connexin 40gap junction proteinvascular cell adhesion molecule 1acute heart infarctionadultArticlecontrolled studyDNA purificationenzyme linked immunosorbent assayfemalegene frequencygene linkage disequilibriumgenetic predispositiongenetic variabilitygenotypehumanmajor clinical studymalepolymerase chain reactionrestriction fragment length polymorphismsingle nucleotide polymorphismbloodcase control studyEgyptgenetic predispositiongeneticsheart infarctionmiddle agedrisk factorAdultCase-Control StudiesConnexinsEgyptFemaleGene FrequencyGenetic Predisposition to DiseaseHumansLinkage DisequilibriumMaleMiddle AgedMyocardial InfarctionPolymerase Chain ReactionPolymorphism, Single NucleotideRisk FactorsVascular Cell Adhesion Molecule-1Articlehttps://doi.org/10.2174/1389201018666171002125432PubMed ID 28969560