Akram El-Meligy, MariamIshaq Bolis, SaraBahaa El-Dein, SherineSameh Mahdy, Taghreed2019-10-132019-10-132019Copyright © 2019 MSA University. All Rights Reserved.https://t.ly/5XPe1Wrinkles are creases, folds, or ridges in the skin. After the age of 20, the skin begins to lose over time its elasticity, firmness and collagen fibers. Accordingly, wrinkles tend to occur in the areas of muscle contraction. Citrus sinensis L. belonging to family Rutaceae are widely consumed in Egypt. Citrus peels are the most important by-product of Citrus fruits. They are major sources of phenolics specially flavanones and their glycosides, which are a powerful natural antioxidant that may possess an anti-wrinkles activity. The aim of this project is to characterize the major metabolites in the 95% ethanolic extract of C. sinensis fruits peels and evaluate its antioxidant and anti-wrinkles activities. The HPLC analysis of the extract revealed that the major compounds were hesperidin and quercetin. The extract was characterized also via HPLC-MS-MS, the analysis revealed that sesamolinol and hesperidin were the major identified constituents. The antioxidant activity was evaluated using ORAC assay and the extract showed promising antioxidant activity. The in vitro anti-wrinkles activity of the extract was evaluated by screening their inhibitory activity on elastase and collagenase enzymes, the extract showed good inhibitory activity with IC50 values of 299.04 ug/ml and 188.6lug/ml respectively, which is close to the tested standards Elafin and EGCG (IC50 250.09 and 112.12 ug/ml). Thus, C. sinensis fruits peels extract can be used to develop a completely natural anti-aging herbal product with antioxidantenOctober University for Modern Sciences and ArtsUniversity of Modern Sciences and ArtsMSA Universityجامعة أكتوبر للعلوم الحديثة والآدابPharmacognosyNANO-FORMULATIONOther