Bakr R.O.El Bishbishy M.H.Pharmacognosy DepartmentFaculty of PharmacyOctober University for Modern Sciences and Arts (MSA)GizaEgypt2020-01-092020-01-0920160102695Xhttps://doi.org/10.1016/j.bjp.2016.04.001PubMed ID :https://t.ly/dOgp6ScopusThe present study was designed to investigate polyphenolic and sulphur contents of the aerial parts of Capparis spinosa var.aegyptia (Lam.) Boiss., Capparaceae, wildly growing in Egypt.The chemical compositions of the water distilled essential oil were investigated by GC/MS analysis where the major constituent of the oil was methyl isothiocyanate (24.66%).Hydroethanolic extract was evaluated by LC-HRESI-MS-MS in both positive and negative modes.Forty-two compounds were identified including quercetin, kaempferol and isorhamnetin derivatives in addition to myricetin, eriodictyol, cirsimaritin and gallocatechin derivatives.Quercetin tetrahexoside dirhamnoside as well as kaempferol dihexoside dirhamnoside have not been identified before in genus Capparis.Phenolic acids, such as quinic acid, p-coumaroyl quinic acid and chlorogenic acid were also identified.Evaluation of cytotoxic activity of hydroethanolic extract against three human cancer cell lines (MCF-7; breast adenocarcinoma cells, Hep-G2; hepatocellular carcinoma cells and HCT-116; colon carcinoma) using 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay showed significant effect with IC50 values 24.5, 24.4 and 11 g/ml, compared to Doxorubicin as a standard cytotoxic drug.C.spinosa revealed itself as a promising candidate for nutraceutical researches. 2016 Sociedade Brasileira de Farmacognosia.EnglishCapparis spinosaCytotoxicityFlavonoidsIsothiocyanateLC-HRESI-MS-MS4 coumaroyl quinic acidabsintholcamphorCapparis spinosa extractcaryophyllenechlorogenic acidcirsimaritincopaenedoxorubicineriodictyolessential oilgallocatechingamma muuroleneglucosinolatehumuleneisopropyl isothiocyanateisorhamnetinkaempferolkaempferol dihexoside dirhamnosidemethyl isothiocyanatemyricetinpara cymeneplant extractplant medicinal productquercetinquercetin tetrahexoside dirhamnosidequinic acidspathulenolsulforaphaneunclassified drugunindexed drugantineoplastic activityArticleCapparis spinosachemical compositioncollisionally activated dissociationcontrolled studycytotoxicityelectrospray mass spectrometryherbal medicinehigh performance liquid chromatographyhumanhuman cellmass fragmentographyMTT assayplant structuresArticlehttps://doi.org/10.1016/j.bjp.2016.04.001PubMed ID :