Youssif, K.AElshamy, A.MRabeh, M.AGabr, NAfifi, W.MSalem, M.AAlbohy, AAbdelmohsen, U.RHaggag, E.G2020-10-312020-10-3110/22/202023656549https://doi.org/10.1002/slct.202002947https://t.ly/VYm7ScopusThe green synthesis of silver nanoparticles (SNPs) using plant extracts has gained great attention due to its unique properties. So, in this study, the aqueous and n-hexane extracts of Lampranthus coccineus F. Aizoaceae were used for the synthesis of silver nanoparticles. The early signs of SNPs synthesis were detected by a color change from yellow to reddish-brown color. The formation of SNPs was further confirmed using a transmission electron microscope (TEM), UV-Visible spectroscopy, and zeta potential. The TEM analysis of SNPs showed spherical nanoparticles with mean size ranges between 8.55 nm to 16.44 nm for Lampranthus coccineus aqueous and hexane extracts. The UV-Visible spectrophotometric analysis showed an absorption peak at λmax of 417 nm, which confirms the formation of SNPs. Zeta potential results indicated that the particles are negatively charged and fairly stable due to the electrostatic repulsion. The cytotoxic activity of the green synthesized SNPs as well as the four isolated compounds (β-Sitosterol-3-O-β-D-glucoside, P-methoxy benzoic acid, dibutyl phthalate, and dioctyl phthalate) was evaluated using MTT assay against MCF-7, HepG-2, and HCT-116 cell lines. Metabolic profiling of the annotated twelve compounds from the crude extract of L. coccineus. These compounds, along with the four isolated compounds were subjected to molecular docking study. © 2020 Wiley-VCH GmbHen-USCytotoxicityDocking studyLampranthus coccineusMetabolic profilingNanoparticlesArticlehttps://doi.org/10.1002/slct.202002947