Hodhod, Mohamed SGaafar, Abdel-Rhman ZAl Munqedhi, Bandar MElzein, AbdallaAbdelmalik, Abdelmalik M2024-02-182024-02-182024-01https://doi.org/10.1515/chem-2023-0184http://repository.msa.edu.eg/xmlui/handle/123456789/5850The green synthesis pathway for silver nanoparticles (AgNPs) used in bacterial treatment is regarded as crucial because of its cost-effectiveness, nontoxicity, and eco-friendliness. During the present work, the mangliculous marine fungi Amarenographium solium isolated from the Arabian Gulf Coast of Saudi Arabia were utilized for the synthesis of AgNP, through the bio-reduction of aqueous silver nitrate (AgNO3) solution. The success in AgNP synthesis was visually identified by the development of dark brown color in the cell-free filtrate and was further con- firmed by ultraviolet–visible spectroscopy, which showed a peak at 425 nm. The AgNPs produced were further characterized using X-ray diffraction data analysis that proved the bioreduction of silver to 20 nm, and transmission electron microscopy revealed the formation of well-dispersed spherical nanoparticles with an average mean size of 12 nm. The optimization reaction parameters of temperature, pH, and metal salt concentration were carried out and resulted in a combination of 30°C, 7 and 1.5 mM, respectively, for rapid and maximum yield production. The antibacterial activity of the produced nanoparticles was evaluated using the two-fold microdilution method and showed a minimum inhibitory concentration of 9.375 μg/mL of AgNP against multiple drugresistant bacterial strains.enmarine fungi, silver nanoparticles, biosynthesis, characterization, optimization, antibacterial activityArticlehttps://doi.org/10.1515/chem-2023-0184