Al-Olayan E.M.El-Khadragy M.F.Aref A.M.Othman M.S.Kassab R.B.Abdel Moneim A.E.Zoology DepartmentFaculty of ScienceKing Saud UniversityRiyadh 11451Saudi Arabia; Zoology and Entomology DepartmentFaculty of ScienceHelwan UniversityCairo 11795Egypt; Biological Science DepartmentFaculty of DentistryModern Sciences and Arts (MSA) UniversityGiza 12111Egypt; Biochemistry and Molecular Biology DepartmentFaculty of BiotechnologyModern Sciences and Arts (MSA) UniversityGiza 12111Egypt; Experimental Biology DepartmentFaculty of ScienceMasaryk University62500 BrnoCzech Republic; Biochemistry and Molecular Biology DepartmentAsturias Institute of BiotechnologyUniversity of Oviedo33006 OviedoSpain2020-01-092020-01-09201419420900https://doi.org/10.1155/2014/381413PubMed ID : 24876910https://t.ly/LXXGmScopusMSA Google ScholarThe active constituent profile in Cape gooseberry (Physalis peruviana L.) juice was determined by GC-MS. Quercetin and kaempferol were active components in the juice. In this study we have evaluated its potential protective effect on hepatic injury and fibrosis induced by carbon tetrachloride (CCl4). Twenty-eight rats divided into 4 groups: Group I served as control group, and Group II received weekly i.p. injection of 2 mL CCl4/kg bwt for 12 weeks. Group III were supplemented with Physalis juice via the drinking water. The animals of Group IV received Physalis juice as Group III and also were intraperitoneally injected weekly with 2 mL CCl4/kg bwt for 12 weeks. Hepatoprotective effect was evaluated by improvement in liver enzymes serum levels, reduction in collagen areas, downregulation in expression of the fibrotic marker MMP-9, reduction in the peroxidative marker malonaldehyde and the inflammatory marker nitric oxide, and restoration of the activity of antioxidant enzymatic and nonenzymatic systems, namely, glutathione content, superoxide dismutase, catalase, glutathione-S-transferase, glutathione peroxidase, and glutathione reductase activities. The results show that the potential hepatoprotective effects of Physalis peruviana may be due to physalis acts by promotion of processes that restore hepatolobular architecture and through the inhibition of oxidative stress pathway. � 2014 Ebtisam M. Al-Olayan et al.EnglishOctober University for Modern Sciences and ArtsUniversity of Modern Sciences and ArtsMSA Universityجامعة أكتوبر للعلوم الحديثة والآدابCarbon tetrachlorideFlavonoidsNitric oxideOxygenPeptidesPlants (botany)RestorationActive constituentsGlutathione contentsGlutathione peroxidaseGlutathione reductaseGlutathione-S-transferaseHepatoprotective effectsProtective effectsSuper oxide dismutaseRatsalanine aminotransferaseantioxidantaspartate aminotransferasecarbon tetrachloridecatalasegelatinase Bglutathioneglutathione peroxidaseglutathione reductaseglutathione transferaseliver protective agentmalonaldehydenitric oxidePhysalis peruviana extractplant extractquercetinsuperoxide dismutaseunclassified druggelatinase Bmalonaldehydenitric oxideoxidoreductaseplant extractprotective agentalanine aminotransferase blood levelanimal experimentanimal modelanimal tissueantioxidant activityarticleaspartate aminotransferase blood levelcarbon tetrachloride-induced liver fibrosiscontrolled studydown regulationdrug effectenzyme activityenzyme repressionfruit juiceimmunohistochemistryin vitro studylipid peroxidationliver function testliver homogenateliver protectionmalemass fragmentographymedicinal plantnonhumanoxidative stressphysalis peruvianaprotein expressionratreal time polymerase chain reactionanimalbloodchemistrydrug effectsDrug-Induced Liver InjurylivermetabolismpathologyPhysalistoxicityWistar ratAnimalsCarbon TetrachlorideDown-RegulationDrug-Induced Liver InjuryLiverLiver Function TestsMaleMalondialdehydeMatrix Metalloproteinase 9Nitric OxideOxidative StressOxidoreductasesPhysalisPlant ExtractsProtective AgentsRatsRats, WistarArticlehttps://doi.org/10.1155/2014/381413PubMed ID : 24876910