Eid, OmneyaEzzat, ShahiraElkady, Wafaa MEl Sayed, AbeerAbd el‑sattar, Essam2024-05-242024-05-242024-05https://doi.org/10.1186/s43094-024-00642-0http://repository.msa.edu.eg/xmlui/handle/123456789/6000Background Cereals have historically played a crucial role in the human diet, serving as a signifcant natural source of energy and ofering various health benefts. Barley (Hordeum vulgare L.) has been given signifcant attention in recent years due to its exceptional nutritional value, surpassing that of other cereals. The objective of this research is to evaluate the antioxidant activity of various solvent extracts obtained from three diferent barley cultivars. Results The G.136 variety’s acetone extract exhibited the highest level of antioxidant activity in both the DPPH assay, with an ­IC50 of 55.62 µg/ml, and the FRAP assay, with 447 μM trolox/mg extract. The dominant compounds identi‑ fed before in the acetone fraction were subjected to an evaluation of their docking scores, along with an assessment of ADMET (Absorption, Distribution, Metabolism, Excretion, and Toxicity) and TOPKAT (Toxicity Prediction by Komputer Assisted Technology) studies. Notably, hordatine A1, prodelphinidin B3, hordatine B1, procyanidin B2, and isovitexin 7-O-glucoside were the major compounds with the highest LipDock scores compared to trolox the reference drug with polyphenol oxidase. Conclusions The fndings indicate that the acetone extract from all three cultivars demonstrates noteworthy results, surpassing the efcacy of other solvent extracts against the antioxidant activity.enAntioxidant, Barley, Cereals, Docking, Hordeum vulgare LArticlehttps://doi.org/10.1186/s43094-024-00642-0