Aboushousha T.Helal N.Hammam O.Ibrahim M.Khaled S.Mostafa A.Anas A.Department of PathologyTheodor Bilharz Research InstituteImbabaGizaEgypt; Faculty of BiotechnologyOctober University of Modern Sciences and ArtsGizaEgypt; Department of SurgeryTheodor Bilharz Research InstituteImbabaGizaEgypt; Department of Hepato-GastroenterologyTheodor Bilharz Research InstituteGizaEgypt2020-01-092020-01-09201818579655https://doi.org/10.3889/oamjms.2018.338https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6236038/ScopusBACKGROUND: Globally, gastric cancer (GC) it is the fourth most common cancer and the third cause of cancer-related deaths. Overexpression of MDM2 and B-RAF appeared to be increased in malignancy and associated with poor prognosis in several human tumours, but their role in gastric cancer remains controversial. AIM: We had investigated the immunohistochemical expression of MDM2 and B-RAF in 136 gastric lesions with/without H. pylori association. MATERIAL AND METHODS: Studied specimens include chronic gastritis (32), intestinal type GC (70), diffuse GC (22) and gastrointestinal stromal tumours (GIST) (12). RESULTS: MDM2 expression increased significantly in intestinal GC compared to other groups (p < 0.001), while B-RAF expression increased significantly in GIST compared to other groups (p < 0.001). H. pylori increased expression of MDM2 in intestinal GC cases but did not affect B-RAF expression. MDM2 expression correlated with high grade of tumor differentiation (p < 0.001), deep invasion (p < 0.05), nodal metastases (p < 0.05) and distant metastases (p < 0.1) in intestinal GC, while B-RAF expression did not correlate with TNM stage (p < 0.1). CONCLUSION: MDM2 up-regulation was more frequent in intestinal GC, while B-RAF up-regulation was more frequent in GIST compared to other groups; MDM2 expression in intestinal GC was correlated with H. pylori association, high grade of differentiation, deep invasion, nodal and distant metastases, meanwhile, B-RAF expression was correlated with high-grade intestinal GC but did not correlate with H. pylori or TNM stage. The possible role of both MDM2 and B-RAF in predicting progression of gastric tumours and prognosis deserves further investigations. 2018 Tarek Aboushousha, Noha Helal, Olfat Hammam, Manar Ibrahim, Samar Khaled, Amr Mostafa, Amgad Anas.EnglishOctober University for Modern Sciences and Artsجامعة أكتوبر للعلوم الحديثة والآدابUniversity of Modern Sciences and ArtsMSA UniversityB-RAFGastric cancerGastritisH. pyloriMDM2B Raf kinaseprotein MDM2Articlechronic gastritisdistant metastasisfemalegastrointestinal stromal tumorHelicobacter pylorihumanhuman tissueimmunohistochemistrymaleprotein expressionstomach lesionstomach tumortumor differentiationArticlehttps://doi.org/10.3889/oamjms.2018.338