Marmouzi, Ilias Ezzat, Shahira MMostafa, Eman Sherien El Jemli, Meryem Radwan, Rasha Ali Faouzi, My El Abbes Tamsouri, Naoufal Kharbach, Mourad 2021-01-292021-01-2912/22/2020https://doi.org/10.1007/s42452-020-04052-8http://repository.msa.edu.eg/xmlui/handle/123456789/4406Despite the richness and biodiversity of invertebrates and algae in the Mediterranean Sea, these organisms are still poorly studied. The objective of our research is the discovery of bioactive lead compounds from the Mediterranean Sea sponge Hemimycale Collumella (HC). HC sponge (189.0  g) was collected from Mdiq costs on the Mediterranean Sea and extracted with methanol to yield (10  g) which was then subjected to fractionation. A bio-guided protocol was applied through evaluation of 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging and Oxygen Radical Absorbance Capacity (ORAC), α-amylase, β-glucosidase, pancreatic lipase inhibition as well as anti-collagenase, anti-elastase, antityrosinase and cytotoxic activity. 2,3-O-Hexahydroxydiphenoyl-(α/β)-glucose (1) and gentisic acid 2-O-β-glucoside (2) were isolated from the water fraction, quercetin-3-O-β-glucopyranoside (3), kaempferol 3-O-β-glucopyranoside (4) and isorhamnetin 3-O-β-glucopyranoside (5) from n-butanol fraction, gallic acid (6) from ethyl acetate fraction and gallic acid-3-methyl ether (7) from methylene chloride fraction. Compound 5 had the highest DPPH and ORAC activity. Compounds 1–5 had promising lipase inhibition activities which exceeded that of the standard Orlistat, while compounds 1–7 showed anti-tyrosinase activity higher than that of the standard Hydroquinone monomethyl ether. This is the first report for evaluation of the biological activities of 2, 3-O-hexahydroxydiphenoyl-(α/β)-glucose (1), gentisic acid 2-O-β-glucoside (2) and gallic acid-3-methyl ether (7).en-USGallic acid-3-methyl etherGallic acid Hemimycale collumella2,3-O-hexahydroxydiphenoyl-(α/β)-glucose Quercetin-3-O-β-glucopyranosideArticlehttps://doi.org/10.1007/s42452-020-04052-8