Dkhil M.A.Al-Quraishy S.Aref A.M.Othman M.S.El-Deib K.M.Abdel Moneim A.E.Department of ZoologyCollege of ScienceKing Saud UniversityRiyadh 11451Saudi Arabia; Department of Zoology and EntomologyFaculty of ScienceHelwan UniversityCairo 11795Egypt; Biological Science DepartmentFaculty of DentistryModern Science and Arts University (MSA)Giza 12111Egypt; Biochemistry and Molecular Biology DepartmentFaculty of PharmacyModern Science and Arts (MSA)Giza 12111Egypt; Molecular Drug Evaluation DepartmentNational Organization for Drug Control and Research (NODCAR)Giza 12553Egypt; Department of Biochemistry and Molecular BiologyAsturias Institute of BiotechnologyUniversity of OviedoOviedo 33006Spain2020-01-092020-01-09201319420900https://doi.org/10.1155/2013/741817PubMed ID : 24369490https://t.ly/DXXAYScopusMSA Google ScholarAzadirachta indica A. Juss. (neem, family: Meliaceae) is perhaps the most commonly used traditional medicinal plant of India. In this study we investigated the protective effect of methanolic neem leaves extract (MNLE; 500 mg/Kg bwt) on rats treated with cisplatin (CDDP)-induced hepatotoxicity. Adult rats were randomly divided into four groups. CDDP was given to rats by intraperitoneal injection, while MNLE was given by oral gavage for 5 days after the CDDP injection. The injury and oxidative stress caused by CDDP on the liver and the effect of MNLE were evaluated by measuring (a) histological changes, (b) tissue biochemical oxidant and antioxidant parameters, and (c) investigating apoptosis markers immunohistochemically and by real time PCR. After treatment with MNLE, the histological damage and apoptosis induction caused by cisplatin were improved. Malondialdehyde and nitric oxide were significantly decreased; the antioxidant system, namely, glutathione content, glutathione-S-transferase, glutathione peroxidase, catalase, and superoxide dismutase activities were significantly elevated. In conclusion, MNLE may have a potential role when combined with cisplatin in chemotherapy to alleviate cisplatin-induced damage and oxidative stress in liver. 2013 Mohamed A. Dkhil et al.Englishantioxidantcaspase 3caspase 9catalasecisplatinimmunoglobulin enhancer binding proteinmessenger RNAplant extractprotective agentprotein Baxsuperoxide dismutaseanimalarticleAzadirachta indicachemistrydrug effectenzymologyfemalegene expression regulationgeneticslipid peroxidationlivermetabolismoxidative stresspathologyphytotherapyplant leafrattoxic hepatitisWistar ratAnimalsAntioxidantsAzadirachtabcl-2-Associated X ProteinCaspase 3Caspase 9CatalaseCisplatinDrug-Induced Liver InjuryFemaleGene Expression RegulationLipid PeroxidationLiverNF-kappa BOxidative StressPhytotherapyPlant ExtractsPlant LeavesProtective AgentsRatsRats, WistarRNA, MessengerSuperoxide DismutaseArticlehttps://doi.org/10.1155/2013/741817PubMed ID : 24369490